Identification of Listeria monocytogenes from unpasteurized apple juice using rapid test kits
| Autor: | Curtis J. Omiecinski, Susan M. Sorg, Patricia N. Sado, Gary J. Husby, Karen C. Jinneman |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Salmonella Rhamnose Pasteurization Food Contamination medicine.disease_cause Microbiology Hemolysis Polymerase Chain Reaction law.invention Beverages chemistry.chemical_compound fluids and secretions Listeria monocytogenes law Multiplex polymerase chain reaction medicine Food science Bacteriological Techniques biology Sterilization Hydrogen-Ion Concentration biology.organism_classification medicine.disease Culture Media Fecal coliform chemistry Fruit Listeria Reagent Kits Diagnostic Food Science |
| Zdroj: | Journal of food protection. 61(9) |
| ISSN: | 0362-028X |
| Popis: | A microbiological survey of 50 retail juices was conducted in the fall of 1996. These juices were analyzed for Listeria monocytogenes, Escherichia coli O157:H7, Salmonella, coliforms, fecal coliforms, and pH. Two unpasteurized juices were positive for L. monocytogenes: an apple juice and an apple raspberry blend with a pH of 3.78 and 3.75, respectively. Three L. monocytogenes isolates were characterized. The colonies were typical for Listeria sp. on Oxford and lithium chloride-phenylethanol-moxalactam agars and were beta-hemolytic on sheep blood agar. The isolates required 5 days of incubation at 35 degrees C to produce a positive rhamnose reaction in a phenol red carbohydrate broth. This slow rhamnose utilization resulted in these isolates not being identified using the Micro-ID test strip (Organon Technika). However, the isolates were positive for L. monocytogenes using the API Listeria strip (BioMerieux) and a multiplex polymerase chain reaction for detection of the hemolysis (hyla) and invasion-associated protein (iap) genes. |
| Databáze: | OpenAIRE |
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