Oxidative stress enhances the expression of sulfur assimilation genes: preliminary insights on the Enterococcus faecalis iron-sulfur cluster machinery regulation
| Autor: | Christine Garcia Bierhals, Gustavo Pelicioli Riboldi, Jeverson Frazzon, Pedro Alves d'Azevedo, Ana Paula Guedes Frazzon, Eduardo Preusser de Mattos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Iron-Sulfur Proteins Models Molecular lcsh:Arctic medicine. Tropical medicine Operon Firmicutes lcsh:RC955-962 lcsh:QR1-502 Iron–sulfur cluster Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Enterococcus faecalis [Fe-S] cluster assembly lcsh:Microbiology Microbiology Substrate Specificity chemistry.chemical_compound suf operon Sulfur assimilation medicine oxidative stress Escherichia coli Articles biology.organism_classification Biosynthetic Pathways chemistry iron depletion Biogenesis Bacteria |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 109, Iss 4, Pp 408-413 (2014) Memórias do Instituto Oswaldo Cruz, Volume: 109, Issue: 4, Pages: 408-413, Published: 06 JUN 2014 Memórias do Instituto Oswaldo Cruz |
| ISSN: | 1678-8060 |
| Popis: | The Firmicutes bacteria participate extensively in virulence and pathological processes. Enterococcus faecalis is a commensal microorganism; however, it is also a pathogenic bacterium mainly associated with nosocomial infections in immunocompromised patients. Iron-sulfur [Fe-S] clusters are inorganic prosthetic groups involved in diverse biological processes, whose in vivo formation requires several specific protein machineries. Escherichia coli is one of the most frequently studied microorganisms regarding [Fe-S] cluster biogenesis and encodes the iron-sulfur cluster and sulfur assimilation systems. In Firmicutes species, a unique operon composed of the sufCDSUB genes is responsible for [Fe-S] cluster biogenesis. The aim of this study was to investigate the potential of the E. faecalis sufCDSUB system in the [Fe-S] cluster assembly using oxidative stress and iron depletion as adverse growth conditions. Quantitative real-time polymerase chain reaction demonstrated, for the first time, that Gram-positive bacteria possess an OxyR component responsive to oxidative stress conditions, as fully described for E. coli models. Likewise, strong expression of the sufCDSUB genes was observed in low concentrations of hydrogen peroxide, indicating that the lowest concentration of oxygen free radicals inside cells, known to be highly damaging to [Fe-S] clusters, is sufficient to trigger the transcriptional machinery for prompt replacement of [Fe-S] clusters. |
| Databáze: | OpenAIRE |
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