Cold-Active Xylanase Produced by Fungi Associated with Antarctic Marine Sponges
| Autor: | Jaime Eyzaguirre, Gloria Levicán, Renato Chávez, Inmaculada Vaca, María Cristina Ravanal, Pamela Ubilla, Abdiel Del-Cid, Exequiel Medina |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Aquatic Organisms
Antarctic Regions Bioengineering Applied Microbiology and Biotechnology Biochemistry Hydrolysis Botany Enzyme Stability Animals Molecular Biology Thermostability chemistry.chemical_classification Endo-1 4-beta Xylanases biology Bran Temperature food and beverages General Medicine Straw Hydrogen-Ion Concentration biology.organism_classification Xylan Porifera Cold Temperature Molecular Weight Enzyme chemistry Xylanase Xylans Cladosporium Biotechnology |
| Zdroj: | Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Despite their potential biotechnological applications, cold-active xylanolytic enzymes have been poorly studied. In this work, 38 fungi isolated from marine sponges collected in King George Island, Antarctica, were screened as new sources of cold-active xylanases. All of them showed xylanase activity at 15 and 23 °C in semiquantitative plate assays. One of these isolates, Cladosporium sp., showed the highest activity and was characterized in detail. Cladosporium sp. showed higher xylanolytic activity when grown on beechwood or birchwood xylan and wheat bran, but wheat straw and oat bran were not so good inducers of this activity. The optimal pH for xylanase activity was 6.0, although pH stability was slightly wider (pH 5–7). On the other hand, Cladosporium sp. showed high xylanase activity at low temperatures and very low thermal stability. Interestingly, thermal stability was even lower after culture media were removed and replaced by buffer, suggesting that low molecular component(s) of the culture media could be important in the stabilization of cold-active xylanase activity. To the best of our knowledge, this study is the first report on extracellular xylanase production by fungi associated with Antarctic marine sponges. |
| Databáze: | OpenAIRE |
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