Effects of actinomycin D on developing hamster molar tooth germs in vitro
| Autor: | D.M. Lyaruu, J.H.M. Wöltgens, Antonius L.J.J. Bronckers, T.J.M. Bervoets, M. A. van Duin |
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| Rok vydání: | 1997 |
| Předmět: |
Hamster
Biology Organ culture Tritium Phosphorus metabolism Organ Culture Techniques stomatognathic system Amelogenesis Cricetinae Ameloblasts Animals Trichloroacetic Acid Dental Enamel General Dentistry Antibiotics Antineoplastic DNA synthesis Dose-Response Relationship Drug Odontoblasts Calcium Radioisotopes Tooth Germ Phosphorus DNA Organ Size Molecular biology Molar Odontoblast Solubility Immunology Toxicity Dactinomycin Odontogenesis Calcium Ameloblast Phosphorus Radioisotopes Cell Division Tooth Calcification Thymidine |
| Zdroj: | European journal of oral sciences. 105(1) |
| ISSN: | 0909-8836 |
| Popis: | The aim of this study was to evaluate the toxic effects of actinomycin D on the developing hamster tooth germ in organ culture. Hamster tooth germs during early secretory amelogenesis were exposed in vitro for 24 h to 10(-9) M-5 x 10(-5) M actinomycin D. Actinomycin D dose-dependently (> or = 10(-7) M) decreased the tooth germ dry weight but mineralization was affected only by doses > or = 10(-5) M. However, the uptakes of TCA-insoluble 32P and [3H]thymidine were significantly reduced dose-dependently from > or = 10(-8) M actinomycin D, indicating that the drug inhibits the synthesis of phosphate-containing macromolecules as well as DNA synthesis. Histologically, 10(-8) M actinomycin D was the lowest dose which was not toxic to any cell type in the developing tooth germ. At 10(-7) M actinomycin D, the most sensitive cells were the proliferating pre-odontoblasts followed by pre-ameloblasts; the mature secretory ameloblasts and odontoblasts appeared unaffected. Higher doses resulted in increased cytotoxicity to the secretory cells and, eventually, total degeneration of most cells. The data suggest that children treated for cancer during tooth development using anti-chemotherapy cocktails containing actinomycin D (serum levels > 10(-7) M) may develop defects later on in the mature dentition as a direct consequence of the toxicity of the drug to the tooth organ. |
| Databáze: | OpenAIRE |
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