The Role of TNF-α induced MSCs on Suppressive Inflammation by Increasing TGF-β and IL-10
| Autor: | Agung Putra, Ika Rosdiana, Ken Wirastuti, Fatkhan Baitul Ridwan, Nur Anna Chalimah Sadyah, Allisha Irwaniyanti Putridewi, Azizah Retno Kustiyah, Delfitri Munir |
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| Rok vydání: | 2018 |
| Předmět: |
TGF-β
0301 basic medicine business.industry lcsh:R Mesenchymal stem cell lcsh:Medicine Inflammation Stimulation General Medicine Pharmacology Phenotype MSC 03 medical and health sciences Interleukin 10 030104 developmental biology Basic Science TNF-α IL-10 TGF beta signaling pathway medicine Tumor necrosis factor alpha medicine.symptom business Transforming growth factor |
| Zdroj: | Open Access Macedonian Journal of Medical Sciences; Vol 6 No 10 (2018): Oct 25 (OAMJMS); 1779-1783 Open Access Macedonian Journal of Medical Sciences Open Access Macedonian Journal of Medical Sciences, Vol 6, Iss 10 (2018) |
| ISSN: | 1857-9655 |
| DOI: | 10.3889/oamjms.2018.404 |
| Popis: | BACKGROUND: Mesenchymal stem cells (MSCs) may serve as immunoregulators by producing various anti-inflammatory molecules. Under sufficient level of TNF-α, MSCs become activated and adopt immune-suppressive phenotype (MSCs type-2) by releasing various anti-inflammatory molecule including TGF-β and IL-10. However, the ability of MSC itself to produce IL-10 under TNF-α stimulation and the correlation of TGF-β production of MSCs to IL-10 level remains to be elucidated. AIM: In this study, MSCs were activated with various TNF-α doses to determine the increase of IL-10 and TGF-β level as well as its correlation. MATERIAL AND METHODS: This study used post-test only control group design, by using 3 study groups, consist of 1 control (C) and 2 treatments (T) (TNF-α = 5 and 10 ng/mL) with triplicate induced in MSC for 24 hours, then the levels of IL-10 and TGF-β were measured by using ELISA assay. RESULTS: The results of this study showed a significant increase of TGF-β and IL-10 levels (p < 0.05) at TNF-α 5 and 10 ng/mL dose of TNF-α. Moreover, there was a significant negative correlation between TGF-β and IL-10 level on 5 and 10 ng/mL dose TNF-α treatment. CONCLUSION: Based on our study, we conclude that the 5 ng/mL dose of TNF-α is a sufficient dose for MSCs to suppress the inflammatory milieu. The higher increase of TGF beta is due to the controlled inflammation by IL-10. |
| Databáze: | OpenAIRE |
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