Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania
| Autor: | Luminita Marutescu, Roxana Maria Nemes, Otilia Banu, Marcela Popa, Irina Gheorghe, Elvira Borcan, Mariana Carmen Chifiriuc, Mihaela Magdalena Mitache, Elena Rusu, Miruna Predescu |
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| Rok vydání: | 2018 |
| Předmět: |
Resistance (ecology)
Materials Science (miscellaneous) Process Chemistry and Technology General Engineering Virulence General Chemistry General Medicine biochemical phenomena metabolism and nutrition Biology General Biochemistry Genetics and Molecular Biology Microbiology Materials Chemistry General Pharmacology Toxicology and Pharmaceutics Gram |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 2668-8212 0034-7752 |
| Popis: | We aimed to investigate the antibiotic resistance�and virulence�markers in�Gram negative bacilli (GNB) and Gram positives coccus (GPC), strains recently isolated from the�hospital environment and from patients with surgical wound infections in order to obtain epidemiologically relevant data.The strains identification was performed with�the automated miniApi system. The resistance�phenotypes were established�using disk diffusion (CLSI, 2017). 61 strains were screened for the production of enzymatic soluble virulence factors: hemolysins, amylase, caseinase, aesculin hydrolysis, DNA-ase, lipase, gelatinase and lecithinase, which give microorganisms the ability to colonize and disseminate in the host. Multiplex PCR reactions were performed for the detection of carbapenemases, aminoglycoside-resistant determinants (AME�s), quinolone and tetracycline resistance in GNR and SCCmec cassette type in�Staphylococcus aureus�strains and�to identify the genetic�support of cell-associated�and soluble virulence factors�in�E. coli�strains�(fimH, sfaDE, papC, eaea, cnf1, bfpa, eaf, AggR, EaggE�genes) and biofilm production in Acinetobacter baumannii isolates (OmpA).The isolated�E. coli and A. baumannii strains were resistant to �-lactam antibiotics, including penicillins and beta-lactamase inhibitors, third / fourth generation cephalosporins and carbapenems (encoded by�blaOXA-48like�and blaTEMlike genes), quinolones (qnrA and qnrB), aminoglycosides�(aadB), and�tetracyclines (encoded by tetA and tetB). Most of the strains presented at least two of the eight tested virulence factors.�The carbapenemases and ESBLs producers proved to be positive for the majority of the tested soluble virulence factors, proving the pathogenic potential of these strains. In�S. aureus�isolates�the molecular analysis showed that 60% of the isolates were MRSA and the molecular analysis revealed the presence of the SCCmec cassette type�mec IVa and III types. Our data suggest the hypothesis according to which nosocomial origin of the strains can be explained by multiple drug resistance and virulence determinants. |
| Databáze: | OpenAIRE |
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