A novel reaction-based fluorescent probe for the detection of cysteine in milk and water samples

Autor: Liu Yongguo, Shaoxiang Yang, Hongyu Tian, Sun Baoguo, Wang Jialin, Wang Hao, Hao Yanfeng
Rok vydání: 2018
Předmět:
Zdroj: Food Chemistry. 262:67-71
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2018.04.084
Popis: A novel fluorescent probe 3′-hydroxy-3-oxo-3H-spiro [isobenzofuran-1,9′-xanthene]-6′-yl-2,4-dinitrobenzenesulfonate (probe 1) was designed and synthesized as a visual sensor for the detection of cysteine levels in milk and water samples. The addition of cysteine to the solution of probe 1 resulted in an increase in fluorescence intensity and color change, from light yellow to yellow-green. The distinct color response indicated that probe 1 could be used as a visual sensor for cysteine. Cysteine can be detected quantitatively at concentrations between 0 and 400 μM and the detection limit of the fluorescence response to the probe was 6.5 μM. This suggests that probe 1 could be used as a signaling tool to determine the cysteine levels in samples, such as milk and water.
Databáze: OpenAIRE