Long-term effect of 2-deoxy-2-fluoroglucose on maintenance in culture of the neonatal B cell of rat
| Autor: | Shohei Kagawa, Akira Matsuoka, Shizuo Shimizu, Keiko Murakoso, Keiko Yoshida, Keiko Nakao, Kunio Haito |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.medical_treatment Biophysics Deoxyglucose Biology Biochemistry Islets of Langerhans chemistry.chemical_compound Biosynthesis Fluorodeoxyglucose F18 Internal medicine Deoxy Sugars Insulin Secretion Cyclic AMP medicine Animals Insulin Term effect Molecular Biology Cells Cultured B cell geography geography.geographical_feature_category Glucose responsiveness Cell Biology Islet Rats Kinetics medicine.anatomical_structure Endocrinology Animals Newborn chemistry 2-fluoroglucose Proinsulin |
| Zdroj: | Biochemical and Biophysical Research Communications. 108:279-285 |
| ISSN: | 0006-291X |
| Popis: | This report describes a beneficial role for 2-deoxy-2-fluoroglucose(2-dfg) in promoting survival of neonatal B cells of the rat. Culturing of islets in medium with 5.5 mM glucose abolished the glucose responsiveness of B cells after 7 days of culture. By contrast, the 2-dfg supplemented culture maintained glucose-induced insulin release, and the recovery of insulin in B cells at day 7 at levels 4 to 7-fold higher than at day 0. Slightly enhanced insulin biosynthesis was observed in those B cells. In addition, a selective deletion of fibroblasts was caused by the addition of the fluoroglucose. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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