Infectious Molecular Clone of a Recently Transmitted Pediatric Human Immunodeficiency Virus Clade C Isolate from Africa: Evidence of Intraclade Recombination
| Autor: | Charles E. Wood, Ruth M. Ruprecht, Lan Yu Yeh, Ganapati Bhat, Weidong Xu, Ricky D. Grisson, Jun He, Chipepo Kankasa, Agnès Laurence Chenine |
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| Rok vydání: | 2004 |
| Předmět: |
clone (Java method)
viruses Molecular Sequence Data Immunology Zambia HIV Infections Biology Microbiology Virus Cell Line Phylogenetics Virology Humans Amino Acid Sequence Cloning Molecular Clade Peptide sequence Phylogeny Recombination Genetic Genetics Strain (biology) Infant Sequence Analysis DNA biology.organism_classification Insect Science Lentivirus HIV-1 Recombination and Evolution Recombination |
| Zdroj: | Journal of Virology. 78:14066-14069 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.78.24.14066-14069.2004 |
| Popis: | Although human immunodeficiency virus type 1 (HIV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir. 15: 1321-1324, 1999; T. Ndung'u, B. Renjifo, and M. Essex, J. Virol. 75: 4964-4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an R5, non-syncytium-inducing isolate that bears all known clade C signatures; gag , pol , and env consistently mapped within clade C. Interestingly, gag resembled Asian isolates, whereas pol and env resembled African isolates, indicating that HIV1084i probably arose from an intraclade recombination. As a recently transmitted clade C strain, HIV1084i will be a useful vaccine development tool. |
| Databáze: | OpenAIRE |
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