Zebrafish HSC70 promoter to express carp muscle-specific creatine kinase for acclimation under cold condition
| Autor: | Chih-Lu Wu, Hsi-Wen Sun, Cho-Fat Hui, Ta-Hui Lin, Tien-Lin Chang, Jen-Leih Wu |
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| Rok vydání: | 2011 |
| Předmět: |
Carps
Embryo Nonmammalian animal structures Microinjections Acclimatization Transgene Danio Transfection Animals Genetically Modified Gene product Common carp Escherichia coli Genetics Animals Transgenes Promoter Regions Genetic Carp Creatine Kinase Zebrafish Swimming biology fungi HSC70 Heat-Shock Proteins Wild type Zebrafish Proteins biology.organism_classification Molecular biology Cold Temperature Enzyme Activation Gene Expression Regulation Models Animal embryonic structures biology.protein Animal Science and Zoology Creatine kinase Agronomy and Crop Science Plasmids Biotechnology |
| Zdroj: | Transgenic Research. 20:1217-1226 |
| ISSN: | 1573-9368 0962-8819 |
| Popis: | Zebrafish (Danio rerio) is used as a model system for in vivo studies. To expand the research scope of physical, biochemical and physiological studies, a cold-tolerant model of zebrafish was developed. The common carp (Cyprinus carpio) muscle form of creatine kinase (CK, EC 2.7.3.2) can maintain enzymatic activity at a temperature of around 15°C. However, a cold-inducible promoter of zebrafish, hsc 70 (heat shock protein 70 cognate), is able to increase the expression of gene product by 9.8 fold at a temperature of 16°C. Therefore, the carp CK gene was promoted by hsc 70 and transfected into zebrafish embryos. Resulting transgenic zebrafish survived and could maintain its swimming behavior at 13°C, which was not possible with the wild-type zebrafish. The swimming distance of the transgenic fish was 42% greater than that of the wild type at 13°C. This new transgenic fish model is ideal for studies of ectothermal vertebrates in low-temperature environments. |
| Databáze: | OpenAIRE |
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