A pi form of glutathione-S-transferase is a myelin- and oligodendrocyte-associated enzyme in mouse brain
| Autor: | Francine A. Tansey, Wendy Cammer |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Blotting Western Fluorescent Antibody Technique Biochemistry Cellular and Molecular Neuroscience Myelin Mice Mice Neurologic Mutants medicine Pi Animals Myelin Sheath Glutathione Transferase chemistry.chemical_classification biology Staining and Labeling Brain Rat brain Molecular biology Immunohistochemistry Oligodendrocyte Enzyme assay Rats Isoenzymes Oligodendroglia medicine.anatomical_structure Enzyme Glutathione S-transferase nervous system chemistry biology.protein Hybridization Genetic Immunostaining |
| Zdroj: | Journal of neurochemistry. 57(1) |
| ISSN: | 0022-3042 |
| Popis: | The pi form of glutathione-S-transferase (GST), previously found to be oligodendrocyte associated, has also been found in myelin. In the brains of adult mice, immunocytochemical staining for a pi form of GST was observed in myelinated fibers, as well as oligodendrocytes. In contrast, and as previously found in rats, positive immunostaining for mu forms occurred in astrocytes and not in oligodendrocytes or myelinated fibers. Double immunofluorescence staining strengthened the conclusion that pi was in oligodendrocytes and myelin in mouse brains. The results of enzyme assays performed on brain homogenates and purified myelin indicated that GST specific activities in mouse brain myelin were almost as high (0.8-fold) as those in mouse brain homogenates. Low, but reproducible, GST activities were also observed in myelin purified from rat brains, in which pi had been demonstrated in oligodendrocytes and mu in astrocytes. The pi form was also stained by the immunoblot technique in myelin purified from mouse brain. It was concluded that pi is a myelin associated, as well as oligodendrocyte associated, enzyme in mouse brain, and possibly also in rat brain. This is the first report of localization of GSTs in mouse brain and of GST in myelin. |
| Databáze: | OpenAIRE |
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