Anticarcinogenic Effect and Enhancement of Metastatic Potential of BALB/c 3T3 Cells by Ginsenoside Rh2
| Autor: | Masayo Maeda, Takahide Ota, Masaaki Tatsuka |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cdk2
Cancer Research Ginsenosides Mice Nude Apoptosis Protein Serine-Threonine Kinases medicine.disease_cause Chemoprevention Article 3T3 cells Transformation Metastasis Oncogene Protein pp60(v-src) Mice chemistry.chemical_compound Ginseng CDC2-CDC28 Kinases Tumor Cells Cultured medicine Animals Anticarcinogenic Agents Neoplastic transformation Neoplasm Metastasis Cell Line Transformed Dose-Response Relationship Drug biology Cyclin-Dependent Kinase 2 Cyclin-dependent kinase 2 3T3 Cells Saponins Molecular biology Cyclin-Dependent Kinases In vitro Cell Transformation Neoplastic medicine.anatomical_structure Oncology chemistry Ginsenoside Cell culture Immunology biology.protein Carcinogenesis Cell Division Neoplasm Transplantation Methylcholanthrene |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| DOI: | 10.1111/j.1349-7006.2001.tb02138.x |
| Popis: | It has been reported that ginsenoside Rh(2), a purified ginseng saponin with a dammarane skeleton, has anticarcinogenic effects on mammalian cells. To determine the significance of these effects on multistage carcinogenesis, we utilized the BALB / c 3T3 cell system. In an in vitro two-stage neoplastic transformation assay, the initiating activity of 3-methylcholanthrene (3-MCA) was suppressed by Rh(2) (>or= 1 x 10(-5) M) in both BALB / c 3T3 A31-1-1 cells and the more carcinogen-susceptible variant A31-1-13 cells. The suppressive effects in this concentration range were thought to be caused by suppression of DNA replication via indirect Cdk2 inhibition. On the other hand, the promotion steps of both the target cells were not affected by Rh(2) even if the transformation frequency was enhanced by a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA). To examine the other effects of Rh(2) on carcinogenesis, we turned our attention to the metastatic phenotype. Using metastatic src-transformed A31-1-1 cells, we found that Rh(2) augmented the metastatic potential in an experimental metastasis assay. These data indicate that Rh(2) has diverse effects on the expression of the transformed phenotype in BALB / c 3T3 cells, but support the idea that growth suppression is likely to be a major mechanism of the anticarcinogenic effects of Rh(2). |
| Databáze: | OpenAIRE |
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