Enhancement of Ethanol Production in Electrochemical Cell by Saccharomyces cerevisiae (CDBT2) and Wickerhamomyces anomalus (CDBT7)
| Autor: | Pranita Poudyal, Rocky Maharjan, Bikram Prajapati, Amar Prasad Yadav, Lakshmaiah Sreerama, Tribikram Bhattarai, Mukesh Yadav, Pradip Dhungana, Jarina Joshi, Milan Mainali |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Economics and Econometrics
Wickerhamomyces anomalus 020209 energy Energy Engineering and Power Technology Lignocellulosic biomass lcsh:A Wickerhamomyces anomalous 02 engineering and technology Saccharomyces cerevisiae Hydrolysate chemistry.chemical_compound 0202 electrical engineering electronic engineering information engineering Ethanol fuel Food science lignocellulosic biomass bioethanol Saccharum spontaneum Ethanol Renewable Energy Sustainability and the Environment Chemistry Substrate (chemistry) 021001 nanoscience & nanotechnology Yeast electrochemical cell Fuel Technology Biofuel lcsh:General Works 0210 nano-technology |
| Zdroj: | Frontiers in Energy Research, Vol 7 (2019) |
| Popis: | Bioethanol (a renewable resource), blended with gasoline, is used as liquid transportation fuel worldwide and produced from either starch or lignocellulose. Local production and use of bioethanol supports local economies, decreases country's carbon footprint and promotes self-sufficiency. The latter is especially important for bio-resource-rich land-locked countries like Nepal that are seeking alternative transportation fuels and technologies to produce them. In that regard, in the present study, we have used two highly efficient ethanol producing yeast strains, viz., Saccharomyces cerevisiae (CDBT2) and Wickerhamomyces anomalous (CDBT7), in an electrochemical cell to enhance ethanol production. Ethanol production by CDBT2 (anodic chamber) and CDBT7 (cathodic chamber) control cultures, using 5% glucose as substrate, were 12.6 ± 0.42 and 10.1 ± 0.17 mg·mL−1 respectively. These cultures in the electrochemical cell, when externally supplied with 4V, the ethanol production was enhanced by 19.8 ± 0.50% and 23.7 ± 0.51%, respectively, as compared to the control cultures. On the other hand, co-culturing of those two yeast strains in both electrode compartments resulted only 3.96 ± 0.83% enhancement in ethanol production. Immobilization of CDBT7 in the graphite cathode resulted in lower enhancement of ethanol production (5.30 ± 0.82%), less than free cell culture of CDBT7. CDBT2 and CDBT7 when cultured in platinum nano particle coated platinum anode and neutral red-coated graphite cathode, respectively, ethanol production was substantially enhanced (52.8 ± 0.44%). The above experiments when repeated using lignocellulosic biomass hydrolysate (reducing sugar content was 3.3%) as substrate, resulted in even better enhancement in ethanol production (61.5 ± 0.12%) as compared to glucose. The results concluded that CDBT2 and CDBT7 yeast strains produced ethanol efficiently from both glucose and lignocellulosic biomass hydrolysate. Ethanol production was enhanced in the presence of low levels of externally applied voltage. Ethanol production was further enhanced with the better electron transport provision i.e., when neutral red was deposited on cathode and fine platinum nanoparticles were coated on the platinum anode. Scopus |
| Databáze: | OpenAIRE |
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