Bone Marrow Lacks a Transplantable Progenitor for Smooth Muscle Type α-Actin–Expressing Cells
| Autor: | Jian Xing Ma, Jen Yue Tsai, Paul W. Kincade, Sanai Sato, Yutaka Kawakami, Yoshinori Nagai, Takafumi Yokota |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Stromal cell
Green Fluorescent Proteins Myocytes Smooth Muscle Mice Transgenic Biology Muscle Smooth Vascular Mice Bone Marrow medicine Animals Myocyte Bone Marrow Transplantation Transplantation Chimera Transdifferentiation Hematopoietic Stem Cell Transplantation Cell Biology Hematopoietic Stem Cells Molecular biology Actins Cell biology Mice Inbred C57BL Endothelial stem cell Haematopoiesis medicine.anatomical_structure Mesangial Cells Molecular Medicine Bone marrow Stromal Cells Stem cell Developmental Biology Adult stem cell |
| Zdroj: | Stem Cells. 24:13-22 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1634/stemcells.2004-0346 |
| Popis: | While some studies have suggested that hematopoietic stem cells might give rise to other tissue types, others indicate that transdifferentiation would have to be an extremely rare event. We have now exploited smooth muscle type α-actin (αSMA) promoter– driven green fluorescent protein (GFP) transgenic mice (αSMA-GFP mice) for bone marrow transplantation to evaluate their potential to generate donor-type tissues in irradiation chimeras. There was a highly restricted pattern of GFP expression in the transgenic mice, marking bone marrow stromal cells and mesangial cells in the kidney. However, these characteristics were not transferable to wild-type animals given transgenic marrow cells even though hematopoietic cells were largely replaced. Our findings support earlier studies suggesting that the bone marrow microenvironment is difficult to transplant and indicate that hematopoietic stem cells are unlikely to give rise to αSMA-expressing progeny. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text