Calditerricola satsumensis gen. nov., sp. nov. and Calditerricola yamamurae sp. nov., extreme thermophiles isolated from a high-temperature compost
| Autor: | Yusuke Terui, Isao Yumoto, Takashi Ito, Tairo Oshima, Toshiyuki Moriya, Tomohisa Hikota, Akihiko Yamagishi |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Hot Temperature Molecular Sequence Data medicine.disease_cause Microbiology Planifilum yunnanense DNA Ribosomal chemistry.chemical_compound Soil Valine RNA Ribosomal 16S medicine Polyamines Cluster Analysis Bacillaceae Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Base Composition Methionine biology Thermophile Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Culture Media chemistry Isoleucine Leucine Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 61(Pt 3) |
| ISSN: | 1466-5034 |
| Popis: | Two novel thermophilic micro-organisms, designated YMO81T and YMO722T, were isolated from a high-temperature compost (internal temperature >95 °C). The isolates were able to grow at 80 °C in a nutrient broth and in a synthetic medium. Cells were aerobic, Gram-negative rods (0.3×4.0 μm). Spore formation was not observed. Strain YMO81T grew at 83 °C and pH 6.9–8.9 and grew optimally at 78 °C and pH 7.5 with 2 % NaCl. For growth in a synthetic minimal medium at 70 °C, the vitamins biotin, folic acid and thiamine and the amino acids glutamine and methionine were essential for growth of both strains; at 80 °C, strain YMO81T also required histidine, isoleucine, leucine, lysine, phenylalanine, serine, tryptophan and valine. Cellular fatty acids of the isolates comprised mainly iso-C17 : 0 and anteiso-C17 : 0. The DNA G+C contents of strains YMO81T and YMO722T were 70 and 64 mol%, respectively. When the 16S rRNA gene sequences of the isolates were compared with those of other bacteria, highest similarity was observed with Planifilum yunnanense LA5T (90 % 16S rRNA gene sequence similarity). DNA–DNA relatedness between strain YMO722T and strain YMO81T was 55 %. N 4-Aminopropylspermine was identified as a major polyamine, which suggested that the isolates were distinct from other related taxa. On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, we propose a new genus, Calditerricola gen. nov., and two novel species, the type species Calditerricola satsumensis sp. nov., with type strain YMO81T (=ATCC BAA-1462T =JCM 14719T =DSM 45223T), and Calditerricola yamamurae sp. nov., with type strain YMO722T (=ATCC BAA-1461T =JCM 14720T =DSM 45224T). |
| Databáze: | OpenAIRE |
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