Enhanced cholinergic-tone during the stress induce a depressive-like state in mice
| Autor: | Jean Pierre Oses, Erikson Kaszubowski, André P. Koth, Carolina David Wiener, Marcos F. Cordeiro, Carolina S. Peixoto, Daniela M. Barros, Gustavo M. Parfitt, Andrea F. Soubhia, Fernanda Pedrotti Moreira, Sara S. Fernandes |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Dominance-Subordination Male medicine.medical_specialty Elevated plus maze Anhedonia Hydrocortisone Physostigmine Hippocampus Anxiety Motor Activity Open field Social defeat 03 medical and health sciences Behavioral Neuroscience chemistry.chemical_compound Random Allocation 0302 clinical medicine Helplessness Learned Internal medicine Medicine Animals Prefrontal cortex Depressive Disorder business.industry Acetylcholinesterase Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology chemistry Cholinergic Cholinesterase Inhibitors medicine.symptom business 030217 neurology & neurosurgery Stress Psychological |
| Zdroj: | Behavioural brain research. 347 |
| ISSN: | 1872-7549 |
| Popis: | Major depressive disorder has a heterogeneous etiology, since it arises from the interaction of multiple factors and different pathophysiological mechanisms are involved in the symptomatology. This study aimed to investigate the role of the cholinergic system in the susceptibility to stress and, consequently, in the depression-like behavior. C57BL/6 mice were treated with Physostigmine (PHYS), an acetylcholinesterase (AChE) inhibitor, and were submitted to the social defeat stress. For the behavioral evaluation of the locomotor activity, anxiety-like and depression-like behaviors the open field, elevated plus maze, sucrose preference, social interaction and forced swim were used. Hippocampus and prefrontal cortex samples were collected for evaluation of AChE activity, as well as blood samples for analysis of serum cortisol levels. Our results showed that 15 min after the injection of PHYS there was a significant inhibition of AChE activity in the hippocampus and in the prefrontal cortex. On the other hand, in the end of the experimental design, day 12, there was no difference in AChE activity levels. Inhibition of AChE and exposure to the stress led to an increase in cortisol levels. Animals that received PHYS and were exposed to stress showed less social interaction and greater learned helplessness, anhedonia and anxious-like behavior. Taken together, our findings suggest that increasing the cholinergic tone shortly before stress induction impacts on the ability to cope with upcoming stressful situations, leading to a depressive-like state. |
| Databáze: | OpenAIRE |
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