A low cost fermentation medium for potential fibrinolytic enzyme production by a newly isolated marine bacterium, Shewanella sp. IND20☆
| Autor: | S.G. Prakash Vincent, Ponnuswamy Vijayaraghavan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Optimization
Central composite design lcsh:Biotechnology Shewanella sp Applied Microbiology and Biotechnology Shewanella Article Fibrinolytic enzyme chemistry.chemical_compound Response surface methodology lcsh:TP248.13-248.65 Food science ComputingMethodologies_COMPUTERGRAPHICS biology Substrate (chemistry) Factorial experiment biology.organism_classification Trehalose Thrombolytic agent Solid-state fermentation Biochemistry chemistry Fermentation Biotechnology |
| Zdroj: | Biotechnology Reports Biotechnology Reports, Vol 7, Iss C, Pp 135-142 (2015) |
| ISSN: | 2215-017X |
| Popis: | Graphical abstract Agro-residues were used as the substrate for the production of fibrinolytic enzyme in solid state fermentation. In this study, two-level full factorial design (25) and response surface methodology were applied to optimize a fermentation medium for the production of fibrinolytic enzyme from the marine isolate Shewanella sp. IND20. The 25 factorial design demonstrated that the physical factors (pH and moisture) and nutrient factors (trehalose, casein, and sodium dihydrogen phosphate) had significant effect on fibrinolytic enzyme production. Central composite design was employed to search for the optimal concentration of the three factors, namely moisture, pH, and trehalose, and the experimental results were fitted with a second-order polynomial model at 99% level (p |
| Databáze: | OpenAIRE |
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