Effects of an ethanolic extract and fractions from Piper glabratum (Piperaceae) leaves on pain and inflammation
| Autor: | Joyce Alencar Santos Radai, Jonas da Silva Mota, Fábio Juliano Negrão, Idalina Cristina Ferrari, Maicon Matos Leitão, Candida Aparecida Leite Kassuya, Saulo Euclides Silva-Filho, Rodrigo Juliano Oliveira |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Leukocyte migration Anti-Inflammatory Agents 010501 environmental sciences Toxicology 030226 pharmacology & pharmacy 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Phytol Mice 0302 clinical medicine Chondrus Toxicity Tests Acute Animals Edema Rats Wistar 0105 earth and related environmental sciences Analgesics Stigmasterol biology Traditional medicine Dose-Response Relationship Drug Ethanol Plant Extracts General Medicine Piperaceae biology.organism_classification Acute toxicity Carrageenan Rats Plant Leaves chemistry Phytochemical Hyperalgesia Toxicity Female Piper |
| Zdroj: | Regulatory toxicology and pharmacology : RTP. 117 |
| ISSN: | 1096-0295 |
| Popis: | In the state of Mato Grosso do Sul, Brazil, Piper glabratum leaves are used as a popular medicine for pain and inflammation. We performed a phytochemical analysis and evaluated the effects of ethanolic extract (EEPG) obtained from leaves of P. glabratum on toxicity as well as the effects of application of the hexanic fraction (HXPG) and the hydroalcoholic fraction (HAPG) obtained from the EEPG on inflammatory parameters and pain in mice. Swiss mice were treated with EEPG (30–300 mg/kg body weight (b.w.)), HXPG (19.5 mg/kg b.w.) or HAPG (83.37 mg/kg b.w.) and then subjected to carrageenan-induced pleurisy and paw oedema tests, the spontaneous pain, and zymosan-induced intra-articular inflammation. Wistar rats were treated with EEPG to assess acute toxicity. Phytochemical analysis of the fractions demonstrated the presence of phytol and mixture of stigmasterol and β-sitosterol in the fractions. In the acute toxicity test, LD50 above 2000 mg/kg b.w. was observed. The treatments reduced oedema, cold and mechanical hyperalgesia, leukocyte migration and protein exudation. The antihyperalgesic and anti-inflammatory properties of EEPG and fractions were demonstrated in the present study. These results from EEPG and HXPG may be related, at least in part, to modulation of the inflammatory mediators by phytol, stigmasterol and β-sitosterol. |
| Databáze: | OpenAIRE |
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