Yellow fever outbreak, Imatong, southern Sudan
| Autor: | Fredrick A. Okoth, Norman C. Waters, Samson L. Konongoi, Rodney L. Coldren, Felicity J. Burt, Robert Swanepoel, Rosemary Sang, Peter M. Tukei, Victor Ofula, Kevin M. De Cock, Abdourahmane Sow, Clayton Onyango |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Male
Epidemiology viruses lcsh:Medicine Antibodies Viral Disease Outbreaks Sudan Mice Seroepidemiologic Studies Chlorocebus aethiops Child biology Reverse Transcriptase Polymerase Chain Reaction Yellow fever Brain Amplicon Middle Aged Vaccination Flavivirus Infectious Diseases Child Preschool RNA Viral ELISA Biological Assay Female Yellow fever virus Microbiology (medical) Adult Adolescent Virus lcsh:Infectious and parasitic diseases Yellow Fever medicine Animals Humans lcsh:RC109-216 Vero Cells Research lcsh:R amplicon Outbreak nucleotide sequence Sequence Analysis DNA biology.organism_classification medicine.disease Virology Animals Newborn Oligonucleotide primer Vero cell |
| Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, Vol 10, Iss 6, Pp 1064-1068 (2004) |
| ISSN: | 1080-6040 |
| Popis: | In May 2003, the World Health Organization received reports about a possible outbreak of a hemorrhagic disease of unknown cause in the Imatong Mountains of southern Sudan. Laboratory investigations were conducted on 28 serum samples collected from patients in the Imatong region. Serum samples from 13 patients were positive for immunoglobulin (Ig) M antibody to flavivirus, and serum samples from 5 patients were positive by reverse transcription–polymerase chain reaction with both the genus Flavivirus reactive primers and yellow fever virus–specific primers. Nucleotide sequencing of the amplicons obtained with the genus Flavivirus oligonucleotide primers confirmed yellow fever virus as the etiologic agent. Isolation attempts in newborn mice and Vero cells from the samples yielded virus isolates from five patients. Rapid and accurate laboratory diagnosis enabled an interagency emergency task force to initiate a targeted vaccination campaign to control the outbreak. |
| Databáze: | OpenAIRE |
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