High-Resolution Optical Mapping of Inflammatory Macrophages Following Endovascular Arterial Injury
| Autor: | Farouc A. Jaffer, Charles P. Lin, Jason R. McCarthy, Yoshiko Iwamoto, Amit Saxena, Chase W. Kessinger, Brian Thompson |
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| Rok vydání: | 2012 |
| Předmět: |
Neointima
Cancer Research Pathology medicine.medical_specialty Inflammation Article Mice Restenosis In vivo Image Processing Computer-Assisted Fluorescence microscope Animals Medicine Macrophage Radiology Nuclear Medicine and imaging Cell Proliferation business.industry Macrophages Optical Imaging Endothelial Cells Vascular System Injuries medicine.disease Mice Inbred C57BL Carotid Arteries Microscopy Fluorescence Oncology Immunohistochemistry Endothelium Vascular medicine.symptom Carotid Artery Injuries business Intravital microscopy |
| Zdroj: | Molecular Imaging and Biology. 15:282-289 |
| ISSN: | 1860-2002 1536-1632 |
| Popis: | Inflammation following arterial injury mediates vascular restenosis, a leading cause of cardiovascular morbidity. Here we utilize intravital microscopy (IVM) and a dextran-coated nanosensor to spatially map inflammatory macrophages in vivo following endovascular injury of murine carotid arteries. C57Bl/6 mice (n = 23) underwent endovascular guidewire carotid arterial injury. At day 14 or day 28 post-injury, mice underwent fluorescence IVM, 24 h after injection with the near-infrared fluorescent macrophage nanosensor CLIO-VT680. Adventitial collagen was concomitantly imaged using second harmonic generation (SHG) IVM. Correlative fluorescence microscopy and immunohistochemistry were performed. Two-plane IVM reconstructions detected macrophage inflammation in the arterial wall that was elevated at day 14 compared to day 28 animals (P |
| Databáze: | OpenAIRE |
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