Response of transglutaminase activity and bound putrescine to changes in light intensity under natural or controlled conditions in Quercus ilex leaves
| Autor: | Massimo Zacchini, Marina de Agazio, Maria A. Santos, Marta Pintó-Marijuan, Isabel Fleck, Josep María Torné |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia y Tecnología (España), Universidad de Barcelona |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Chlorophyll
Light Physiology Plant Science chemistry.chemical_compound Quercus Botany Genetics Putrescine Fluorometry Chlorophyll fluorescence Plant Proteins photoperiodism Transglutaminases biology Cell Biology General Medicine biology.organism_classification Fagaceae Spermidine Plant Leaves Light intensity Nylons chemistry Darkness Polyamine |
| Zdroj: | Physiologia plantarum (Online) 131 (2007): 159–169. doi:10.1111/j.1399-3054.2007.00939.x info:cnr-pdr/source/autori:Pintó-Marijuan M.1;de Agazio M.2; Zacchini M.2; M.A. Santos3; J.M. Torné3;Fleck I1/titolo:Response of transgluminase activity and bound putrescine to changes in light intensity under natural or controlled conditions in Quercus ilex L. leaves/doi:10.1111%2Fj.1399-3054.2007.00939.x/rivista:Physiologia plantarum (Online)/anno:2007/pagina_da:159/pagina_a:169/intervallo_pagine:159–169/volume:131 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2005-0399 |
| DOI: | 10.1111/j.1399-3054.2007.00939.x |
| Popis: | In order to further study a previously observed relationship between polyamine (PA) content and changes in irradiation, we examined the level of free and bound PAs, the activity of transglutaminase (TGase, EC 2.3.2.13) and chlorophyll fluorescence in holm oak (Quercus ilex L.) leaves in response to different levels of light intensity and amount. A diurnal trend of free and bound putrescine (F-Put and B-Put, respectively) and TGase activity was observed in plants under natural conditions in the forest, with the highest value corresponding to the maximum light intensity and amount of light received by the leaves. In another set of experiments, potted Q. ilex plants in experimental fields were subjected to a range of periods of natural photosynthetic photon flux density (PPFD) by covering or not covering the whole trees. Under a natural photoperiod (uncovered leaves), B-Put content and TGase activity paralleled the diurnal PPFD pattern, reaching a maximum at the highest PPFD; prior to this maximum, free PAs showed a significant rise. Plants that were in darkness until midday and suddenly exposed to high light intensity showed enhanced TGase activity, resulting in the maximum accumulation of B-Put. The involvement of the accumulation of B-Put reflected in the changes of the B-Put/bound spermidine ratio during the photoprotective responses to high light stress in forest plants is discussed in relation to the chlorophyll fluorescence parameters observed. The research was supported by funds from the Spanish Ministry of Science and Technology research project CGL2005-03998/BOS and research project BF12003-003318. We thank the Servei de Camps Experimentals, Universitat de Barcelona, for technical assistance; Dr I. Casals (Serveis Cientific-Tècnics, Universitat de Barcelona) for helpful discussion; L. Cabañeros and J. Vilamú (Parc de Collserola) for forest facilities; Dr Alex Campos, Dr Xavier Aranda and Ricard Brossa for helpful advice and Robin Rycroft (Servei d’Assesorament Lingüístic, Escola d’Idiomes Moderns, Universitat de Barcelona) for correcting the English manuscript. M. P.-M was a recipient of a doctorate grant from the Universitat de Barcelona. |
| Databáze: | OpenAIRE |
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