HIV-1-Specific CD4+ T-Cell Responses Are Not Associated With Significant Viral Epitope Variation in Persons With Persistent Plasma Viremia
| Autor: | Thomas B. Campbell, Cara C. Wilson, Eric Rapaport, John Koeppe |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male HIV Antigens Molecular Sequence Data HIV Core Protein p24 Epitopes T-Lymphocyte Gene Products gag HIV Infections Biology gag Gene Products Human Immunodeficiency Virus Virus Epitope Interferon-gamma Viral Proteins Immune system Interferon medicine Antigenic variation Humans Pharmacology (medical) Amino Acid Sequence Viremia Peptide sequence Cells Cultured Genetic Variation virus diseases Middle Aged Virology Reverse transcriptase Cross-Sectional Studies Infectious Diseases HIV-1 Female Sequence Alignment CD8 medicine.drug |
| Zdroj: | JAIDS Journal of Acquired Immune Deficiency Syndromes. 41:140-148 |
| ISSN: | 1525-4135 |
| DOI: | 10.1097/01.qai.0000195608.32885.38 |
| Popis: | Objectives: To determine whether increased sequence variation occurs in regions of endogenous HIV-1 targeted by HIV-1-specific CD4 + T cells. The presence of increased variation would be suggestive of immune evasion by HIV-1. Design: We performed a cross-sectional study of untreated HIV-1-infected subjects measuring HIV-1-specific interferon (IFN)-γ-secreting CD4 + T-cell responses against epitopes in Gag p17 and p24 and concurrent endogenous plasma HIV-1 RNA epitope sequence variation. Methods: CD8- depleted IFNγ enzyme-linked immunospot assays were used to identify regions of HIV-1 Gag recognized by CD4 + T cells. Reverse transcriptase polymerase chain reaction and TA cloning were used to sequence endogenous plasma HIV-1 virus and identify variants. Results: CD4 + T-cell epitopes in Gag p17 and p24 were identified in 5 individuals, and concurrent sequence information on endogenous HIV-1 was obtained in 4 of these individuals. Endogenous plasma HIV-1 RNA sequencing revealed no intrapatient amino acid sequence variation through identified epitopes. Conclusions: In these chronically infected viremic subjects, circulating IFNγ-secreting CD4 + T-cell responses were directed against epitope sequences found in the predominant strain of endogenous circulating plasma HIV-1, suggesting that escape from CD4 + T-cell responses is not a common process in vivo. |
| Databáze: | OpenAIRE |
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