In vivo characterization of abdominal aortic aneurysms using an elastin specific molecular MR probe
| Autor: | Simon P. Robinson, Michael S. Marber, Marcus R. Makowski, Christian Jansen, Hans U. Ebersberger, Alice Warley, René M. Botnar, Richard R. Cesati, Andrea J. Wiethoff, Tobias Schaeffter, Reza Razavi, David C. Onthank, Ulrike Blume |
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| Jazyk: | angličtina |
| Předmět: |
Medicine(all)
medicine.medical_specialty Pathology Radiological and Ultrasound Technology biology business.industry Aortic wall Histological staining In vivo cardiovascular system Medical imaging biology.protein Oral Presentation Medicine Radiology Nuclear Medicine and imaging Cardiology and Cardiovascular Medicine business Nuclear medicine Elastin Angiology |
| Zdroj: | Journal of Cardiovascular Magnetic Resonance |
| ISSN: | 1532-429X |
| DOI: | 10.1186/1532-429x-16-s1-o13 |
| Popis: | Background Rupture of abdominal aortic aneurysms (AAAs) is the third most common cause of death in cardiovascular diseases. Despite this high significance, there is still controversy regarding the management of AAAs, as diameter is currently the only accepted parameter to assess risk of rupture. Elastin is the key protein for maintaining stability of the aortic wall. The aim of this study was to evaluate a novel small-molecular-weight elastin-specific MR probe for the in-vivo assessment of aortic wall integrity in AAAs. Methods ApoE-knockout-mice (ApoE-/-) were infused with angiotensin-II (Ang-II) for up to four weeks (1000 ng/ kg/min) to induce AAA formation. An elastin-specific MR probe (Lantheus Medical Imaging, USA) was administered 1, 2, 3 and 4 weeks following Ang-II infusion. Mice were scanned at each time point pre, post control agent (Gd-DTPA) and after administration of the elastin-specific probe. Imaging was performed using a 3T Philips-Achieva MR-scanner equipped with a microscopy coil. Imaging parameters of 3D IR-MRI: spatial resolution = 0.1 × 0.1 mm, 0.5 mm slice-thickness, TR/ TE = 28/8.2 ms. Additionally 3D-T1 mapping was performed. Ex-vivo tissue samples were analysed by inductively-coupled-plasma mass-spectroscopy (ICP-MS), histological staining and electron microscopy. Results |
| Databáze: | OpenAIRE |
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