| Autor: |
Robert, Jérôme, Button, Emily, Stukas, Sophie, Boyce, Guilaine, Ebrima Gibbs, Cowan, Catherine, Gilmour, Megan, Cheng, Wai, Soo, Sonja, Yuen, Brian, Bahrabadi, Arvin, Kang, Kevin, Kulic, Iva, Francis, Gordon, Cashman, Neil, Wellington, Cheryl |
| Rok vydání: |
2017 |
| DOI: |
10.6084/m9.figshare.c.3860566_d6 |
| Popis: |
Adhesion molecules are enhanced by TNF-α but not Aβ.(a-c) hCMEC/D3 were primed with 100 μg/mL HDL for 2 h followed by stimulation with 1 ng/mL TNF-α for 3 h. Cell lysates were prepared in RIPA and protein levels of (a) ICAM-1 and (b) VCAM-1 were measured by denaturing immunoblotting (c). (d-g) hCMEC/D3 were stimulated with monomeric (d,f) Aβ40 or (e,g) Aβ42 at the indicated concentrations and (d,e) ICAM-1 and (f,g) VCAM-1 mRNA levels were measured by real-time PCR. (h-j) Following Aβ stimulation, cell surface proteins were biotinylated and isolated by immunoprecipitation. Protein levels of cell surface and total (h) ICAM-1 and (i) VCAM-1 were measured by denaturing immunoblotting (j). (k-m) hCMEC/D3 were pre-treated with 100 μg/mL of HDL for 2 h followed by stimulation with Aβ. After 3 h total and cell surface ICAM-1 expression were measured as above. Graphs represent means ± SD from at least 3 independent trials. ***p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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