The Nesprin-1/-2 ortholog ANC-1 regulates organelle positioning in C. elegans independently from its KASH or actin-binding domains
| Autor: | Shilpi Kalra, Daniel A. Starr, Leslie A Guerrero, Natalie E. Cain, Hongyan Hao, Laura E Jameson, Jessica Bolivar |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Movement nuclear positioning Science Genetically Modified Cell Cycle Proteins Endoplasmic Reticulum General Biochemistry Genetics and Molecular Biology KASH domains cell biology Organelle Genetics Animals Protein Interaction Domains and Motifs Biology (General) Caenorhabditis elegans Caenorhabditis elegans Proteins Cytoskeleton Actin Cell Nucleus Organelles General Immunology and Microbiology Nesprin Chemistry General Neuroscience Endoplasmic reticulum Microfilament Proteins Calcium-Binding Proteins Nuclear Proteins LINC complexes Lipid Droplets General Medicine nuclear envelope Actins Transmembrane protein Mitochondria Cell biology ER Cytoplasm C. elegans nesprin Medicine Generic health relevance Biochemistry and Cell Biology Signal Transduction Protein Binding |
| Zdroj: | eLife, Vol 10 (2021) |
| Popis: | KASH proteins in the outer nuclear membrane comprise the cytoplasmic half of linker of nucleoskeleton and cytoskeleton (LINC) complexes that connect nuclei to the cytoskeleton. Caenorhabditis elegans ANC-1, an ortholog of Nesprin-1/2, contains actin-binding and KASH domains at opposite ends of a long spectrin-like region. Deletion of either the KASH or calponin homology (CH) domains does not completely disrupt nuclear positioning, suggesting neither KASH nor CH domains are essential. Deletions in the spectrin-like region of ANC-1 led to significant defects, but only recapitulated the null phenotype in combination with mutations in the transmembrane (TM) span. In anc-1 mutants, the endoplasmic reticulum ER, mitochondria, and lipid droplets were unanchored, moving throughout the cytoplasm. The data presented here support a cytoplasmic integrity model where ANC-1 localizes to the ER membrane and extends into the cytoplasm to position nuclei, ER, mitochondria, and other organelles in place. |
| Databáze: | OpenAIRE |
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