The effect of intracerebroventricular injection of beta amyloid peptide (1-42) on caspase-3 activity, lipid peroxidation, nitric oxide and NOS expression in young adult and aged rat brain
| Autor: | Sibel Dinçer, Gonca Akbulut, Nuray Yazihan, Ferihan Cetin |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Aging Amyloid Blotting Western Hippocampus Nitric Oxide Synthase Type II Nitric Oxide Nitric oxide Lipid peroxidation chemistry.chemical_compound Internal medicine Malondialdehyde medicine Animals Rats Wistar Injections Intraventricular Temporal cortex Brain Chemistry Amyloid beta-Peptides business.industry Caspase 3 Brain Glutathione medicine.disease Peptide Fragments Rats Endocrinology chemistry Surgery Neurology (clinical) Lipid Peroxidation Alzheimer's disease business |
| Zdroj: | Turkish neurosurgery. 23(2) |
| ISSN: | 1019-5149 |
| Popis: | Aim Intracerebroventricular (icv) administration of beta amyloid peptide (Aβ) in rats can be used to model certain aspects of Alzheimer disease (AD).The purpose of this study was to examine the effects of intracerebroventricular Aβ (1-42) peptide injection on caspase-3 activity and expression of nNOS and iNOS, malondialdehyde (MDA), glutathione (GSH) and NOx in the hippocampus, temporal cortex and parietal cortex. Material and methods Groups were defined as 1) young adult control, 2) Aβ (1-42) injected young adult, 3) aged control and 4) Aβ (1-42) injected aged group. Stereotaxic surgery was performed. Aβ (1-42) peptide (5μg/1μl, in each icv) was administered bilateral intracerebroventricularly as a single injection. Results Caspase-3 activity significantly increased in Aβ (1-42) injected aged rats when compared with young adult rats. Aβ (1-42) significantly increased lipid peroxidation in both young adult and aged rats. There was an increase in nNOS expression in the temporal cortex of Aβ (1-42) injected aged rats. Conclusion The most significant increase was seen in hippocampus in caspase-3 levels of the Aged- Aβ 1-42 group. nNOS expression in the hippocampus of aged rats was increased compared to young adult rats. However, nNOX expression in the hippocampus of Aβ (1-42) injected aged rats decreased significantly. |
| Databáze: | OpenAIRE |
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