Repression of Pentose Phosphate Pathway Dehydrogenase Synthesis and mRNA by Dietary Fat in Rats
| Autor: | James Tomlinson, Roderick Nakayama, Darold Holten |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Linoleic acid Medicine (miscellaneous) Dehydrogenase Glucosephosphate Dehydrogenase Pentose phosphate pathway chemistry.chemical_compound Dietary Carbohydrates Animals Glucose-6-phosphate dehydrogenase RNA Messenger chemistry.chemical_classification Nutrition and Dietetics biology Chemistry Phosphogluconate Dehydrogenase Body Weight Rats Inbred Strains Metabolism Carbohydrate 5 8 11 14-Eicosatetraynoic Acid Dietary Fats Enzyme assay Rats Gene Expression Regulation Liver Biochemistry Fatty Acids Unsaturated biology.protein Polyunsaturated fatty acid |
| Zdroj: | The Journal of Nutrition. 118:408-415 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/118.3.408 |
| Popis: | We have studied the effects of polyunsaturated fatty acid and its metabolism on the activity, relative synthesis and mRNA levels for rat hepatic glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD). Rats were meal-fed high carbohydrate diets containing either no fat, 5% safflower oil or 5% safflower oil + eicosa-5,8,11,14-tetraynoic acid (TYA). Hepatocytes were isolated and used as a source of RNA, de novo radiolabeled protein and postmitochondrial supernatant for enzyme assay. Dietary safflower oil, as a source of linoleic acid, repressed G6PD activity, synthesis and mRNA levels two- to threefold without significantly changing the amount of carbohydrate consumed. Similar but smaller changes were observed for 6PGD. Dietary fat + TYA (an analogue of arachidonate that inhibits normal metabolism of linoleic acid) prevented the fat-dependent lowering of G6PD and 6PGD activity, synthesis and mRNA levels. Our results suggest that a metabolite of linoleic acid regulates the activity of two lipogenic enzymes, G6PD and 6PGD, by lowering gene expression or mRNA processing or stability. |
| Databáze: | OpenAIRE |
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