Repeated Mild Traumatic Brain Injury Results in Long-Term White-Matter Disruption
| Autor: | Virginia Donovan, Udochuwku Oyoyo, Claudia Kim, Jacqueline S. Coats, Andrea C. Pardo, Andre Obenaus, Ariana K Anugerah |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology Poison control Neurodegenerative Cardiorespiratory Medicine and Haematology Corpus callosum Corpus Callosum Rats Sprague-Dawley Myelin Recurrence Cortex (anatomy) Myelin Sheath Microscopy bilateral injury Trauma Severity Indices diffusion tensor imaging myelin Diffusion Tensor Imaging medicine.anatomical_structure Neurology Neurological Biomedical Imaging Original Article Cardiology and Cardiovascular Medicine medicine.medical_specialty Physical Injury - Accidents and Adverse Effects Traumatic brain injury Clinical Sciences Neuropathology Traumatic Brain Injury (TBI) Electron White matter Microscopy Electron Transmission medicine Transmission Animals Traumatic Head and Spine Injury Neurology & Neurosurgery Animal business.industry Neurosciences medicine.disease Axons Brain Disorders Rats Surgery Disease Models Animal nervous system Brain Injuries Disease Models Sprague-Dawley Neurology (clinical) business axon caliber Diffusion MRI |
| Zdroj: | Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, vol 34, iss 4 |
| ISSN: | 1559-7016 0271-678X |
| Popis: | Mild traumatic brain injury (mTBI) is an increasing public health concern as repetitive injuries can exacerbate existing neuropathology and result in increased neurologic deficits. In contrast to other models of repeated mTBI (rmTBI), our study focused on long-term white-matter abnormalities after bilateral mTBIs induced 7 days apart. A controlled cortical impact (CCI) was used to induce an initial mTBI to the right cortex of Single and rmTBI Sprague Dawley rats, followed by a second injury to the left cortex of rmTBI animals. Shams received only a craniectomy. Ex vivo diffusion tensor imaging (DTI), transmission electron microscopy (TEM), and histology were performed on the anterior corpus callosum at 60 days after injury. The rmTBI animals showed a significant bilateral increase in radial diffusivity (myelin), while only modest changes in axial diffusivity (axonal) were seen between the groups. Further, the rmTBI group showed an increased g-ratio and axon caliber in addition to myelin sheath abnormalities using TEM. Our DTI results indicate ongoing myelin changes, while the TEM data show continuing axonal changes at 60 days after rmTBI. These data suggest that bilateral rmTBI induced 7 days apart leads to progressive alterations in white matter that are not observed after a single mTBI. |
| Databáze: | OpenAIRE |
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