Nanoformulation of paclitaxel to enhance cancer therapy
| Autor: | Jie Chen, James Xing, Xiaojing Zhang, Min Huang, Quanrong Gu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cancer chemotherapy
cell killing Diseases cancer cell culture Pharmacology Mitotic arrest proton nuclear magnetic resonance chemistry.chemical_compound Neoplasms drug uptake drug delivery system Medicine Pluronic copolymers Drug Carriers Copolymers Loading Hydrogels particle size drug distribution Enzyme inhibition Paclitaxel Synthesis (chemical) Drug delivery nanocarrier cancer therapy cytotoxicity nanotoxicology sorbitan palmitate poloxamer HeLa cell scanning electron microscopy in vitro study nanopharmaceutics high performance liquid chromatography Biomedical Engineering Cancer therapy antineoplastic activity nanoencapsulation Biomaterials Cancer Chemotherapy toxicity testing Cell Line Tumor transmission electron microscopy physical chemistry Humans Chemotherapy cell viability phosphate buffered saline Toxicity business.industry water-soluble IC 50 Antineoplastic Agents Phytogenic drug efficacy cell strain MCF 7 drug formulation drug structure Water soluble chemistry Nanoformulation drug solubility Microtubule Inhibitor Nanoparticles aqueous solution business HeLa Cells |
| Zdroj: | Journal of Biomaterials Applications. 28:298-307 |
| ISSN: | 1530-8022 0885-3282 |
| DOI: | 10.1177/0885328212446822 |
| Popis: | Paclitaxel is a microtubule inhibitor causing mitotic arrest and is widely used in cancer chemotherapy. However, its poor water solubility restricts its direct clinical applications. In this article, we report paclitaxel-loaded nanoparticles that are water soluble and that can improve the drug’s bio-distribution and therapeutic efficacy. Paclitaxel-loaded nanoparticles were synthesized by using Pluronic copolymers (F-68 and P-123) and surfactant (Span 40) as nanocarrier. The toxicity and cellular uptake of paclitaxel-loaded nanoparticles were evaluated. The paclitaxel-loaded nanoparticles can completely disperse into phosphate buffer saline to produce a clear aqueous suspension. Based on HPLC analysis, the drug-loading rate is 9.0 ± 0.1% while drug encapsulation efficiency is 99.0 ± 1.0%. The cytotoxicity assay was performed using breast cancer MCF-7 and cervical cancer Hela cells. For MCF-7 cells, the half maximal inhibitory concentrations (IC50) of paclitaxel-loaded nanoparticles and paclitaxel are 8.5 ± 0.3 and 14.0 ± 0.7 ng/mL at 48 hours and 3.5 ± 0.4 and 5.2 ± 0.5 ng/mL at 72 hours across several runs. IC50 of paclitaxel-loaded nanoparticles and paclitaxel for Hela cells are 5.0 ± 0.3 and 8.0 ± 0.3 ng/mL at 48 hours and 2.0 ± 0.1 and 6.5 ± 0.3 ng/mL at 72 hours. In-vitro studies show that the drug’s nanoformulation gives obvious enhancements in the drug’s efficiency at killing cancer cells over paclitaxel alone. Materials of the nanocarrier used for nanoformulation are approved with low toxicity according to the result of cell studies. Conclusion: paclitaxel-loaded nanoparticles greatly improved the physicochemical properties of paclitaxel without modifying its chemical structure, allowing for deep-site cancer drug delivery and enhancing the drug therapeutic efficiency. |
| Databáze: | OpenAIRE |
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