Early differential gene expression in haemocytes from resistant and susceptible Biomphalaria glabrata strains in response to Schistosoma mansoni
| Autor: | Anthony J. Walker, Anne E. Lockyer, Richard A. Kane, Catherine S. Jones, Aidan M. Emery, Christine Coustau, Claus D. Mayer, Leslie R. Noble, Coen M. Adema, Guillaume Mitta, David Rollinson, Ben Hanelt |
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| Přispěvatelé: | Wolfson Wellcome Biomedical Laboratories, The Natural History Museum, School of Biological Sciences, University of Aberdeen, School of Life Sciences, Kingston University, BioSS (Biomathematics & Statistics Scotland) Office (BioSS), Aberdeen University, Ecologie et évolution des interactions (2EI), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Center for Evolutionary and Theoretical Immunology (CETI), The University of New Mexico [Albuquerque], Jones, Catherine S., Ecologie et évolution des interactions [2011-2014] (2EI), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Natural History Museum, Université de Perpignan Via Domitia (UPVD) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Hemocytes
Microarrays Biomphalaria Gene Expression Snail Biomphalaria glabrata 0302 clinical medicine mollusque Gene expression Immune Response Oligonucleotide Array Sequence Analysis 0303 health sciences Parasitologie Multidisciplinary défense cellulaire biology Reverse Transcriptase Polymerase Chain Reaction Malacology Genomics Schistosoma mansoni Host-Pathogen Interaction Immunité adaptative [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology Medicine Disease Susceptibility Research Article Signal Transduction expression des gènes sporocyste Adaptive immunology Science Mechanisms of Resistance and Susceptibility 030231 tropical medicine Immunology réponse immunitaire alliedhealth infection parasitaire Real-Time Polymerase Chain Reaction Microbiology superoxyde dismutase Mollusc defence Host-Parasite Interactions 03 medical and health sciences Expressed sequence tag biology.animal Virology schistosomiasis réponse au stress ubiquinone parasitic diseases Genetics Animals [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology EST RNA Messenger signal de transduction transporteur mitochondrial Biology 030304 developmental biology Schistosoma cDNA microarray Haemocytes Gene Expression Profiling fungi Electron carrier activity Computational Biology biology.organism_classification Molecular biology Immunity Innate Schistosomiasis mansoni Gene expression profiling schistosoma Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology Genome Expression Analysis Zoology biological Biomarkers intéraction hôte parasite |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51102 Plos One 12 (7) (2012) HAL PLoS ONE, Public Library of Science, 2012, 7 (12), ⟨10.1371/journal.pone.0051102⟩ PLoS ONE, Vol 7, Iss 12, p e51102 (2012) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0051102⟩ |
| Popis: | © 2012 Lockyer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The outcome of infection in the host snail Biomphalaria glabrata with the digenean parasite Schistosoma mansoni is determined by the initial molecular interplay occurring between them. The mechanisms by which schistosomes evade snail immune recognition to ensure survival are not fully understood, but one possibility is that the snail internal defence system is manipulated by the schistosome enabling the parasite to establish infection. This study provides novel insights into the nature of schistosome resistance and susceptibility in B. glabrata at the transcriptomic level by simultaneously comparing gene expression in haemocytes from parasite-exposed and control groups of both schistosome-resistant and schistosome-susceptible strains, 2 h post exposure to S. mansoni miracidia, using an novel 5 K cDNA microarray. Differences in gene expression, including those for immune/stress response, signal transduction and matrix/adhesion genes were identified between the two snail strains and tests for asymmetric distributions of gene function also identified immune-related gene expression in resistant snails, but not in susceptible. Gene set enrichment analysis revealed that genes involved in mitochondrial electron transport, ubiquinone biosynthesis and electron carrier activity were consistently up-regulated in resistant snails but down-regulated in susceptible. This supports the hypothesis that schistosome-resistant snails recognize schistosomes and mount an appropriate defence response, while in schistosome-susceptible snails the parasite suppresses this defence response, early in infection. This work was carried out with funding from the Wellcome Trust (068589/Z/02/Z). CMA and BH acknowledge support from the United States National Institutes of Health (NIH), USA R01AI052363 (CMA) and COBRE 1P20RR018754 IdEA, NCRR. |
| Databáze: | OpenAIRE |
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