Characterization and description of a virus causing salivary gland hyperplasia in the housefly, Musca domestica
| Autor: | R R Coler, J H Frank, Drion G. Boucias, Alejandra Garcia-Canedo, James E. Maruniak, J.C. Pendland |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Population Insect Viruses Salivary Glands Virus Houseflies Internal medicine medicine Animals Housefly education Ecology Evolution Behavior and Systematics Gel electrophoresis education.field_of_study General Veterinary Salivary gland biology fungi DNA Viruses DNA virus DNA biology.organism_classification Molecular biology Microscopy Electron medicine.anatomical_structure Endocrinology Insect Science Muscidae DNA Viral Female Parasitology Salivary gland hyperplasia |
| Zdroj: | Medical and Veterinary Entomology. 7:275-282 |
| ISSN: | 1365-2915 0269-283X |
| Popis: | A double-stranded DNA virus was isolated from hyperplasic salivary glands of male and female houseflies, Musca domestica L. (Diptera: Muscidae), collected from a dairy in Alachua County, Florida, U.S.A. Sodium dodecyl sulphate (SDS)–polyacrylamide gel electrophoresis (PAGE) of this housefly salivary gland hyperplasia (SGH) virus revealed the presence of two major and eight minor structural polypeptides. Restriction endonuclease analysis indicated that the c. 137 kilobase pair DNA was double-stranded. Weekly sweep-net sampling of the fly population throughout the season (May-October, 1991) showed that 1.5-18.5% of the dissected flies possessed hyperplasic salivary glands. The virus replicated within the nuclei of the salivary gland cells and was transmitted per os to newly-emerged healthy adult flies. |
| Databáze: | OpenAIRE |
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