Oesophagostomum radiatum: the effect of different adjuvants on vaccination with a partially purified protective antigen
| Autor: | C.J. Fitzgerald, D.A. Berrie, Iain J. East |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_treatment
Freund's Adjuvant Size-exclusion chromatography Antibodies Helminth Cattle Diseases Enzyme-Linked Immunosorbent Assay Fraction (chemistry) Biology Quillaja Saponins Microbiology Feces Animal science Adjuvants Immunologic medicine Animals Parasite Egg Count Polyacrylamide gel electrophoresis General Veterinary Dextran Sulfate Vaccination General Medicine Saponins Weight range Oesophagostomum Protective antigen Antigens Helminth Chromatography Gel Cattle Electrophoresis Polyacrylamide Gel Parasitology Oesophagostomiasis Adjuvant Oesophagostomum radiatum |
| Zdroj: | Veterinary Parasitology. 49:191-200 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/0304-4017(93)90118-7 |
| Popis: | The components of a soluble extract of adult Oesophagostomum radiatum were separated into four fractions by gel filtration chromatography on a Superose-12 column. The second fraction had a molecular weight range of approximately 60 000–65 000 as judged by SDS gel electrophoresis. Vaccination of calves with this fraction significantly (P < 0.05) reduced faecal egg output after challenge infection. In addition, the packed cell volume of vaccinated calves was higher than that of control calves (P < 0.05). The effect of three adjuvants on the vaccine activity was compared. Fraction 2 presented in dextran sulfate reduced the faecal egg count by 93.6% (P < 0.05), Fraction 2 in Freund's complete adjuvant reduced the faecal egg count by 27% (P = N.S.). Quil A was ineffective as an adjuvant. |
| Databáze: | OpenAIRE |
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