High-dose bee venom exposure induces similar tolerogenic B-cell responses in allergic patients and healthy beekeepers
| Autor: | Tadech Boonpiyathad, Andrzej Eljaszewicz, Milena Sokolowska, Norbert Meyer, Oliver F. Wirz, W. van de Veen, M. M. Tomasiak-Lozowska, Marcin Moniuszko, Kiat Ruxrungtham, Anna Bodzenta-Lukaszyk |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Regulatory B cells Immunology B-Lymphocyte Subsets Dose-Response Relationship Immunologic Biology Lymphocyte Activation Immunoglobulin E Immunophenotyping Immune tolerance 03 medical and health sciences Occupational Exposure Hypersensitivity Immune Tolerance medicine Humans Immunology and Allergy IL-2 receptor B cell B-Lymphocytes Environmental Exposure Environmental exposure Allergens Immunoglobulin A Bee Venoms Phospholipases A2 Tolerance induction 030104 developmental biology medicine.anatomical_structure Desensitization Immunologic Immunoglobulin G biology.protein Cytokines Antibody Biomarkers |
| Zdroj: | Allergy. 72:407-415 |
| ISSN: | 0105-4538 |
| DOI: | 10.1111/all.12966 |
| Popis: | Background The involvement of B cells in allergen tolerance induction remains largely unexplored. This study investigates the role of B cells in this process, by comparing B-cell responses in allergic patients before and during allergen immunotherapy (AIT) and naturally exposed healthy beekeepers before and during the beekeeping season. Methods Circulating B cells were characterized by flow cytometry. Phospholipase A2 (PLA)-specific B cells were identified using dual-color staining with fluorescently labeled PLA. Expression of regulatory B-cell-associated surface markers, interleukin-10, chemokine receptors, and immunoglobulin heavy-chain isotypes, was measured. Specific and total IgG1, IgG4, IgA, and IgE from plasma as well as culture supernatants of PLA-specific cells were measured by ELISA. Results Strikingly, similar responses were observed in allergic patients and beekeepers after venom exposure. Both groups showed increased frequencies of plasmablasts, PLA-specific memory B cells, and IL-10-secreting CD73- CD25+ CD71+ BR 1 cells. Phospholipase A2-specific IgG4-switched memory B cells expanded after bee venom exposure. Interestingly, PLA-specific B cells showed increased CCR5 expression after high-dose allergen exposure while CXCR4, CXCR5, CCR6, and CCR7 expression remained unaffected. Conclusions This study provides the first detailed characterization of allergen-specific B cells before and after bee venom tolerance induction. The observed B-cell responses in both venom immunotherapy-treated patients and naturally exposed beekeepers suggest a similar functional immunoregulatory role for B cells in allergen tolerance in both groups. These findings can be investigated in other AIT models to determine their potential as biomarkers of early and successful AIT responses. |
| Databáze: | OpenAIRE |
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