Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases
| Autor: | Tohru Ikegami |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Glycan
medicine.drug_class Filtration and Separation Monoclonal antibody 01 natural sciences Analytical Chemistry 03 medical and health sciences medicine Monosaccharide Solid phase extraction Chromatography High Pressure Liquid 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography biology Hydrophilic interaction chromatography 010401 analytical chemistry 0104 chemical sciences Glycoproteomics Biopharmaceutical Pharmaceutical Preparations chemistry biology.protein Peptides Selectivity Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of Separation Science. 42:130-213 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.201801074 |
| Popis: | Applications of hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs, i.e., glycosylated proteins represented by monoclonal antibodies are discussed in the manner of glycoproteomics. They can be analyzed using hydrophilic interaction chromatography in five different stages as (1) their intact forms, (2) their subunits, (3) N- and O-glycopeptides digested by proteases, (4) N- and O-glycans released from the glycoproteins or glycopeptides, and (5) monosaccharides. Hydrophilic interaction chromatography is a more useful tool in the order of (1) to (5). At the stages (4) and (5), quantitation of glycans and saccharides are also reported. Hydrophilic interaction chromatography is employed not only for analytical uses, but also pretreatment items as solid phase extraction, followed by reversed-phase liquid chromatography separations. Comprehensive search results of these application of hydrophilic interaction chromatography are summarized in tables to show what kind of hydrophilic interaction chromatography columns are suitable for each step of analysis.Relationship of favored and less favored hydrophilic interaction chromatography columns and their separation characteristics such as hydrophilicity, and selectivity for structural difference, is also discussed. Analysis of the therapeutic peptides (not glycosylated) using hydrophilic interaction chromatography is summarized, too. |
| Databáze: | OpenAIRE |
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