Freezing and recovery of hybridoma cell lines
| Autor: | John G.R. Hurrell, Miyoko Takahashi, Steven A. Fuller |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cryopreservation
Hybridomas Cell Antibodies Monoclonal chemical and pharmacologic phenomena General Medicine Biology Liquid nitrogen Freezing methods Cell biology Cell Line Antibody production Hybridoma cell Mice medicine.anatomical_structure Cell culture Immunology Freezing medicine biology.protein Animals Antibody |
| Zdroj: | Current Protocols in Molecular Biology |
| ISSN: | 1934-3647 |
| Popis: | Liquid nitrogen storage is the method of choice for long-term safekeeping of hybridoma cell lines. Frozen aliquots of originally isolated hybridomas provide insurance against loss of antibody production and vigor during culture. There are many variations of cell freezing methods in use. However, for freezing and recovering hybridomas and lymphoid cells in general, the protocol described in this unit is simple and has been successful. |
| Databáze: | OpenAIRE |
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