CBL-interacting protein kinase 25 contributes to root meristem development
| Autor: | Mukesh Kumar Meena, Debasis Chattopadhyay, Vineeta Tripathi, Niraj Kumar Vishwakarma |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Auxin efflux Cytokinins Physiology Meristem Arabidopsis Plant Science Protein Serine-Threonine Kinases Plant Roots 01 natural sciences cytokinin 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Auxin Gene expression heterocyclic compounds Protein kinase A chemistry.chemical_classification Regulation of gene expression Indoleacetic Acids biology Arabidopsis Proteins fungi PIN food and beverages root biology.organism_classification Research Papers SHY2 Cell biology 030104 developmental biology chemistry Cytokinin Growth and Development CIPK25 auxin Signal Transduction 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/ery334 |
| Popis: | CIPK25, a calcium-regulated protein kinase, plays an important role in the co-ordination of auxin and cytokinin signaling in root meristem development. Co-ordination of auxin and cytokinin activities determines root meristem size during post-embryonic development. Calcineurin B-like proteins (CBLs) and their interacting protein kinases (CIPKs) constitute signaling modules that relay calcium signals. Here we report that CIPK25 is involved in regulating the root meristem size. Arabidopsis plants lacking CIPK25 expression displayed a short root phenotype and a slower root growth rate with fewer meristem cells. This phenotype was rescued by restoration of CIPK25 expression. CIPK25 interacted with CBL4 and -5, and displayed strong gene expression in the flower and root, except in the cell proliferation domain in the root apical meristem. Its expression in the root was positively and negatively regulated by auxin and cytokinin, respectively. The cipk25 T-DNA insertion line was compromised in auxin transport and auxin-responsive promoter activity. The cipk25 mutant line showed altered expression of auxin efflux carriers (PIN1 and PIN2) and an Aux/IAA family gene SHY2. Decreased PIN1 and PIN2 expression in the cipk25 mutant line was completely restored when combined with a SHY2 loss-of-function mutation, resulting in recovery of root growth. SHY2 and PIN1 expression was partially regulated by cytokinin even in the absence of CIPK25, suggesting a CIPK25-independent cytokinin signaling pathway(s). Our results revealed that CIPK25 plays an important role in the co-ordination of auxin and cytokinin signaling in root meristem development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|