The response of cementoblasts to calcium phosphate resin-based and calcium silicate-based commercial sealers
| Autor: | Sema S. Hakki, Maria Giovanna Gandolfi, Buket S. Bozkurt, Betül Özçopur, Sema Belli, Carlo Prati |
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| Přispěvatelé: | Hakki SS, Bozkurt BS, Ozcopur B, Gandolfi MG, Prati C, Belli S. |
| Rok vydání: | 2012 |
| Předmět: |
Calcium Phosphates
Time Factors Materials science Serial dilution Cell Survival Cementoblast Osteocalcin Dentistry chemistry.chemical_element Core Binding Factor Alpha 1 Subunit Calcium Real-Time Polymerase Chain Reaction Composite Resins Collagen Type I Cell Line Root Canal Filling Materials Mice calcium silicate Gene expression Animals Integrin-Binding Sialoprotein RNA Messenger Viability assay Aluminum Compounds Cell Shape General Dentistry Dental Cementum biology Epoxy Resins business.industry Silicates viability Temperature cementoblast Humidity Oxides Calcium Compounds Alkaline Phosphatase Molecular biology Resin Cements Drug Combinations Real-time polymerase chain reaction chemistry apatite biology.protein Alkaline phosphatase business Silicate Cement |
| Zdroj: | International Endodontic Journal. 46:242-252 |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/j.1365-2591.2012.02122.x |
| Popis: | Hakki SS, Bozkurt BS, Ozcopur B, Gandolfi MG, Prati C, Belli S. The response of cementoblasts to calcium phosphate resin-based and calcium silicate-based commercial sealers. International Endodontic Journal, 46, 242-252, 2013. Aim To investigate cell viability and gene expression of cementoblasts (OCCM.30) exposed to extractable components released by resin-based sealers with different chemical composition Hybrid Root Seal (HRS), SimpliSeal (SS), Real Seal (RS) and AH Plus (AH) and by a MTA-based sealers Tech Biosealer Endo (TBE). Methodology Discs of all materials were prepared and allowed to set in humid conditions at 37 degrees for 48h. The discs were then incubated for 72h at 37 degrees C to obtain material extracts (1/1) in DMEM. The extracts containing the components released by the sealers were filtered and other dilutions (1/2, 1/4) were prepared from the original solution (1/1). Original and diluted solutions were tested on the cementoblasts. Impedance-based real-time cell analysis (RTCA) was used to evaluate cell viability, quantitative real-time polymerase chain reaction (QRT-PCR) was used to examine the expression of mineralization-related genes (osteocalcin; OCN, Runt-related transcription factor-2; Runx2, collagen type 1; COL I, alkaline phosphatase; ALP). For statistical analysis, one-way analysis of variance (anova) and Tukey's honestly significant difference (HSD) tests were used. Results TBE (1/2), RS (1/2, 1/4), and HRS (1/2, 1/4) significantly decreased cell viability (P |
| Databáze: | OpenAIRE |
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