Chronic inflammation and CD16+ natural killer cell zeta-chain downregulation in hemodialysis patients
| Autor: | Theodoros Eleftheriadis, Dimitra Markala, Charalambos Kartsios, Efi Yiannaki, Vassilios Liakopoulos, Georgia Antoniadi, Polizo Kazila |
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| Rok vydání: | 2007 |
| Předmět: |
Male
CD3 Complex medicine.medical_treatment Receptors Antigen T-Cell Down-Regulation Inflammation CD16 Biology Natural killer cell Flow cytometry Immune system Downregulation and upregulation Renal Dialysis medicine Humans Lymphocyte Count Receptor Aged medicine.diagnostic_test Receptors IgG Hematology General Medicine Middle Aged Flow Cytometry Killer Cells Natural medicine.anatomical_structure Nephrology Case-Control Studies Immunology Chronic Disease Kidney Failure Chronic Female Hemodialysis medicine.symptom Biomarkers |
| Zdroj: | Blood purification. 26(4) |
| ISSN: | 1421-9735 |
| Popis: | Background: Natural killer (NK) cell activity is decreased in hemodialysis (HD) patients. Zeta-chain phosphorylation is an early event that follows the triggering of some NK cell-activating receptors. NK cell zeta-chain is downregulated in patients with cancer due to chronic inflammation. HD is also a chronic inflammatory state. NK cell zeta-chain expression in HD was evaluated. Patients and Methods: Thirty-three HD patients and 30 healthy volunteers were enrolled into the study. The CD3–CD16+ subpopulation was examined, since it corresponds to 90% of all NK cells and has the highest cytotoxicity.NK cell count and zeta-chain mean fluorescence intensity were evaluated with flow cytometry. The inflammatory markers C-reactive protein, IL-6 and tumor necrosis factor-α were measured with ELISA. Results: The inflammatory markers were increased in HD patients. NK cell count did not differ between HD patients and healthy volunteers. NK cell zeta-chain mean fluorescence intensity was decreased in the patient group. Conclusions: Chronic inflammation could be responsible for the NK cell zeta- chain downregulation in HD patients, contributing to the decreased NK cell activity. |
| Databáze: | OpenAIRE |
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