Pericentrin, a highly conserved centrosome protein involved in microtubule organization
| Autor: | Patricia D. Calarco, Louise G. Evans, Stephen J. Doxsey, Marc W. Kirschner, Pascal A. Stein |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Restriction Mapping Fluorescent Antibody Technique Gene Expression Centrosome cycle Spindle Apparatus Aster (cell biology) Biology Autoantigens Microtubules General Biochemistry Genetics and Molecular Biology PCNT Microtubule Tubulin Animals Humans Amino Acid Sequence RNA Messenger Antigens Cloning Molecular Pericentriolar material Microtubule nucleation Centrioles Genetics Microtubule organizing center Cell biology Molecular Weight Microscopy Electron Centrosome Microtubule-Associated Proteins |
| Zdroj: | Cell. 76(4) |
| ISSN: | 0092-8674 |
| Popis: | Antisera from scleroderma patients that react widely with centrosomes in plants and animals were used to isolate cDNAs encoding a novel centrosomal protein. The nucleotide sequence is consistent with a 7 kb mRNA and contains an open reading frame encoding a protein with a putative large coiled-coil domain flanked by noncoiled ends. Antisera recognize a 220 kd protein and stain centrosomes and acentriolar microtubule-organizing centers, where the protein is localized to the pericentriolar material (hence, the name pericentrin). Anti-pericentrin antibodies disrupt mitotic and meiotic divisions in vivo and block microtubule aster formation in Xenopus extracts, but do not block gamma-tubulin assembly or microtubule nucleation from mature centrosomes. These results suggest that pericentrin is a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays. |
| Databáze: | OpenAIRE |
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