Active transport of lead by the calcium pump in human red cell ghosts

Autor:	T. J. B. Simons
Rok vydání:	1988
Předmět:	Calcium metabolism Physiology Chemistry Calcium pump Erythrocyte Membrane Kinetics Biological Transport Active chemistry.chemical_element Calcium Michaelis–Menten kinetics Adenosine Triphosphate Lead Biochemistry Biophysics Humans Vanadate Calcium Channels Efflux Vanadates IC50 Research Article
Zdroj:	The Journal of Physiology. 405:105-113
ISSN:	0022-3751
DOI:	10.1113/jphysiol.1988.sp017323
Popis:	1. Resealed human red cell ghosts containing lead buffers bring about a net transfer of lead from the cell interior to the outside. This transfer is ATP dependent. 2. The active transport of lead is characterized by a Vmax (maximum velocity) of 11 mmol/(l cells.h) and a KM (Michaelis constant) of 5 x 10(-8) M for internal Pb2+, at pH 6.8 and 37 degrees C. 3. Lead efflux is antagonized by internal calcium, and is inhibited by vanadate with the same IC50 (inhibition constant) with which vanadate inhibits calcium pumping. 4. It is concluded that lead is transported by the calcium pump.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34b9f07e0ca8bedeafdf4bf31ae046b1 https://doi.org/10.1113/jphysiol.1988.sp017323 Zobrazit plný text záznamu Plný text