Fiber-based platform for synchronous imaging of endogenous and exogenous fluorescence of biological tissue
| Autor: | Laura Marcu, Cai Li, Diego R. Yankelevich, Julien Bec, Alba Alfonso-Garcia, Ben Sherlock |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Optical fiber Time Factors Bioengineering 02 engineering and technology Optical Physics 01 natural sciences Article Green fluorescent protein law.invention 010309 optics Optics law Clinical Research 0103 physical sciences Animals Fiber Electrical and Electronic Engineering Laser-induced fluorescence Optical Fibers Fluorescent Dyes Quantum Physics Multi-mode optical fiber business.industry Optical Imaging 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics Autofluorescence Optical chopper Cattle Generic health relevance 0210 nano-technology business Pericardium |
| Zdroj: | Opt Lett Optics letters, vol 44, iss 13 |
| ISSN: | 1539-4794 |
| Popis: | Endogenous and exogenous fluorescence emission from biological samples encodes complementary information. Here we report, to the best of our knowledge, the first results from an optical imaging platform with interleaved excitation and detection of exogenous and endogenous fluorescence from tissue samples using a single flexible multimode fiber that delivers the excitation beam and collects the emitted light. A custom-built reflective optical chopper wheel with synchronized rotation temporally multiplexes an autofluorescence lifetime imaging apparatus with an intensity-based fluorescence module tailored to imaging green fluorescent protein. We demonstrate the functionality of such platform imaging dyes of varying fluorescence signatures and resolving cellularized areas on bio-engineered tissue constructs. |
| Databáze: | OpenAIRE |
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