Analysis of the active components and metabolites of Taohong Siwu decoction by using ultra high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Autor:	Ling Xiao, Weidong Chen, Qiuyu Bao, Xianchun Duan, Daiyin Peng, Huan Wu, Lingyu Pan, Can Peng, An Zhou
Rok vydání:	2020
Předmět:	Male Angelica sinensis Active components Filtration and Separation Decoction Mass spectrometry 01 natural sciences Mass Spectrometry Analytical Chemistry Rats Sprague-Dawley Sulfation Benzoquinones Animals Medicine Chinese Traditional Polycyclic Aromatic Hydrocarbons Quadrupole time of flight Chromatography High Pressure Liquid Flavonoids Active ingredient Chromatography biology 010405 organic chemistry Chemistry 010401 analytical chemistry Brain biology.organism_classification Rats 0104 chemical sciences Ultra high performance Drugs Chinese Herbal
Zdroj:	Journal of Separation Science. 43:4131-4147
ISSN:	1615-9314 1615-9306
Popis:	Taohong Siwu Decoction is a classic Chinese medicine prescription for treatment of cerebral ischemia and gynecological diseases. However, the active ingredients of Taohong Siwu Decoction have not been identified. In this study, a ultra performance liquid chromatography quadrupole time-of-flight mass system was used to analyze the active components and metabolites of Taohong Siwu Decoction absorbed into the blood and the brain. A total of 39 active compounds and 90 metabolites were identified in the blood and brain by comparing retention times, accurate masses, fragmentation patterns, and literature data. The results showed that flavonoids (Carthamus tinctorius L), aromatic organic acids, and benzoquinones (Angelica sinensis (Oliv.) Diels and Ligusticumchuanxiong hort) were prominent active ingredients in Taohong Siwu Decoction. Furthermore, hydrolysis, glucuronidation, and sulfation were identified as the main metabolic pathways of Taohong Siwu Decoction in vivo. This was the first study to characterize the active components and metabolites of Taohong Siwu Decoction in the blood and brain using ultra performance liquid chromatography quadrupole time-of-flight mass.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34b7635eafb44ed4136a061f6c6db14d https://doi.org/10.1002/jssc.202000498 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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