DNA from mollusc shell: a valuable and underutilised substrate for genetic analyses
| Autor: | Gert-Jan Jeunen, Erica V. Todd, Sara Ferreira, Catherine J. Collins, Kim Rutherford, Rachael Ashby, Neil J. Gemmell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Nuclear gene Zoology lcsh:Medicine Marine Biology Biology 010603 evolutionary biology 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences Perna canaliculus law Mollusc shell Genetics Gene Molecular Biology Polymerase chain reaction 030304 developmental biology 0303 health sciences General Neuroscience lcsh:R General Medicine Mussel biology.organism_classification Substrate (marine biology) Nuclear DNA Aquaculture Fisheries and Fish Science DNA analysis General Agricultural and Biological Sciences |
| Zdroj: | PeerJ, Vol 8, p e9420 (2020) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | Mollusc shells are an abundant resource that have been long used to predict the structures of ancient ecological communities, examine evolutionary processes, reconstruct paleoenvironmental conditions, track and predict responses to climatic change, and explore the movement of hominids across the globe. Despite the ubiquity of mollusc shell in many environments, it remains relatively unexplored as a substrate for molecular genetic analysis. Here we undertook a series of experiments using the New Zealand endemic greenshell mussel, Perna canaliculus, to explore the utility of fresh, aged, beach-cast and cooked mollusc shell for molecular genetic analyses. We find that reasonable quantities of DNA (0.002–21.48 ng/mg shell) can be derived from aged, beach-cast and cooked mussel shell and that this can routinely provide enough material to undertake PCR analyses of mitochondrial and nuclear gene fragments. Mitochondrial PCR amplification had an average success rate of 96.5% from shell tissue extracted thirteen months after the animal’s death. A success rate of 93.75% was obtained for cooked shells. Amplification of nuclear DNA (chitin synthase gene) was less successful (80% success from fresh shells, decreasing to 10% with time, and 75% from cooked shells). Our results demonstrate the promise of mollusc shell as a substrate for genetic analyses targeting both mitochondrial and nuclear genes. |
| Databáze: | OpenAIRE |
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