Bioorthogonal Cleavage and Exchange of Major Histocompatibility Complex Ligands by Employing Azobenzene-Containing Peptides
| Autor: | Rob Meijers, Julien Lescar, Wim J. E. van Esch, Gijsbert M. Grotenbreg, Manfred Raida, Jiawei Yap, Joanna Ai Ling Choo, Steven H. L. Verhelst, Sock Yue Thong |
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| Přispěvatelé: | School of Biological Sciences |
| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Peptidomimetic Stereochemistry Ligands Cleavage (embryo) Major histocompatibility complex Dithionite Catalysis Epitope Epitopes chemistry.chemical_compound Humans Amino Acid Sequence Peptide sequence HLA-A Antigens biology food and beverages General Medicine General Chemistry Combinatorial chemistry Recombinant Proteins Science::Biological sciences [DRNTU] Azobenzene chemistry biology.protein Bioorthogonal chemistry Peptides Azo Compounds |
| Zdroj: | Angewandte Chemie International Edition. 53:13390-13394 |
| ISSN: | 1433-7851 |
| DOI: | 10.1002/anie.201406295 |
| Popis: | Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene-containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high-throughput studies of disease-specific T cell responses. |
| Databáze: | OpenAIRE |
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