Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors
| Autor: | Hong-Mei Li, Ya-Jie Tang, Jean-François P. Hamel |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cell Survival
Glutamine Biomedical Engineering Continuous stirred-tank reactor Bioengineering Cell Count Mice Bioreactors Antigens Neoplasm Heat shock protein Cell Line Tumor Bioreactor Animals Bioprocess chemistry.chemical_classification Chromatography General Medicine equipment and supplies Oxygen Kinetics Glucose chemistry Cell culture Sarcoma Experimental Industrial and production engineering Saturation (chemistry) Glycoprotein Biotechnology |
| Zdroj: | Bioprocess and biosystems engineering. 32(4) |
| ISSN: | 1615-7605 |
| Popis: | Heat-shock protein glycoprotein (gp96) serves as a natural adjuvant for chaperoning antigenic peptide into the immune surveillance pathway. In our laboratory, MethA tumor cell suspension culture process has been recently developed for gp96 production in spinner flask. In this work, effects of dissolved oxygen tension (DOT) and agitation rate on this process were studied in stirred-tank bioreactor. The optimal conditions for gp96 production were different with those for MethA tumor cell growth. MethA tumor cell growth pattern was not much changed by various levels of DOT and agitation rate, while gp96 biosynthesis was more sensitive to DOT and agitation rate. Compared with 50% of DOT, the production and specific productivity of gp96 was increased by 27 and 66% at 10% of DOT, respectively. Compared with the agitation rate of 100 rpm, the production and volumetric productivity of gp96 was increased by 48 and 144% at the agitation rate of 200 rpm, respectively. Low DOT (i.e., 10% of air saturation) and high agitation rate (i.e., 200 rpm) were identified to be favorable for gp96 biosynthesis. The results of this work might be useful to scale-up the bioprocess into the pilot scale. |
| Databáze: | OpenAIRE |
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