Respiratory pattern and phrenic and hypoglossal nerve activity during normoxia and hypoxia in 6-OHDA-induced bilateral model of Parkinson’s disease
| Autor: | Paweł M. Boguszewski, Katarzyna Kaczyńska, Ilona Joniec-Maciejak, Małgorzata Zaremba, Kryspin Andrzejewski, Monika Jampolska |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Hypoglossal Nerve medicine.medical_specialty Respiratory rate Physiology Dopamine Hypoxic ventilatory response Norepinephrine Adrenergic Agents Internal medicine Animals Medicine Parkinson Disease Secondary Rats Wistar Respiratory system Hypoxia Oxidopamine Phrenic and hypoglossal nerves Original Paper Dopaminergic receptor business.industry Respiration Dopaminergic Brain Parkinson Disease Hypoxia (medical) Rats 6-OHDA rat model Phrenic Nerve Disease Models Animal medicine.anatomical_structure Endocrinology Parkinson’s disease Carotid body medicine.symptom business Hypoglossal nerve Respiratory minute volume |
| Zdroj: | The Journal of Physiological Sciences |
| ISSN: | 1880-6562 1880-6546 |
| Popis: | Respiratory disturbances present in Parkinson’s disease (PD) are not well understood. Thus, studies in animal models aimed to link brain dopamine (DA) deficits with respiratory impairment are needed. Adult Wistar rats were lesioned with injection of 6-hydroxydopamine (6-OHDA) into the third cerebral ventricle. Two weeks after hypoxic test was performed in whole-body plethysmography chamber, phrenic (PHR) and hypoglossal (HG) nerve activities were recorded in normoxic and hypoxic conditions in anesthetized, vagotomized, paralyzed and mechanically ventilated rats. The effects of activation and blockade of dopaminergic carotid body receptors were investigated during normoxia in anesthetized spontaneously breathing rats. 6-OHDA injection affected resting respiratory pattern in awake animals: an increase in tidal volume and a decrease in respiratory rate had no effect on minute ventilation. Hypoxia magnified the amplitude and minute activity of the PHR and HG nerve of 6-OHDA rats. The ratio of pre-inspiratory to inspiratory HG burst amplitude was reduced in normoxic breathing. Yet, the ratio of pre-inspiratory time to total time of the respiratory cycle was increased during normoxia. 6-OHDA lesion had no impact on DA and domperidone effects on the respiratory pattern, which indicate that peripheral DA receptors are not affected in this model. Analysis of monoamines confirmed substantial striatal depletion of dopamine, serotonin and noradrenaline (NA) and reduction of NA content in the brainstem. In bilateral 6-OHDA model changes in activity of both nerves: HG (linked with increased apnea episodes) and PHR are present. Demonstrated respiratory effects could be related to specific depletion of DA and NA. |
| Databáze: | OpenAIRE |
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