Quantitative Analysis of Collagen Network Structure and Fibril Dimensions in Cartilage Repair with Autologous Chondrocyte Transplantation
Autor: | Antti Kaukinen, Mika M. Hyttinen, Heikki J. Helminen, Anna Vasara, Teemu K. Långsjö, Mikko J. Lammi, Ilkka Kiviranta |
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Rok vydání: | 2010 |
Předmět: |
Cartilage
Articular Pathology medicine.medical_specialty Histology Knee Joint Swine Cartilage Stereology Biology Fibril Transplantation Autologous Chondrocyte Transplantation Chondrocytes medicine.anatomical_structure Microscopy Electron Transmission Collagen network medicine Animals Anatomy Cartilage repair Collagen Type II Quantitative analysis (chemistry) Cells Cultured |
Zdroj: | Cells Tissues Organs. 192:351-360 |
ISSN: | 1422-6421 1422-6405 |
DOI: | 10.1159/000319469 |
Popis: | Objective: The aim of this study was to undertake a stereological analysis to quantify the dimensions of the collagen network in the repair tissue of porcine joints after they had been subjected to autologous chondrocyte transplantation (ACT). Method: ACT was used to repair cartilage lesions in knee joints of pigs. Electron-microscopic stereology, immunostaining for type II collagen, and quantitative polarized-light microscopy were utilized to study the collagen fibrils in the repair tissue 3 and 12 months after the operation. Results: The collagen volume density (VV) was lower in the repair tissue than in normal cartilage at 3 months (20.4 vs. 23.7%) after the operation. The collagen surface density (SV, 1.5·10–2 vs. 3.1·10–2 nm2/nm3) and VV increased with time in the repair tissue (20.4 vs. 44.7%). Quantitative polarized-light microscopy detected a higher degree of collagen parallelism in the repair tissue at 3 months after the operation (55.7 vs. 49.7%). In contrast, 1 year after the operation, fibril parallelism was lower in the repair tissue than in the control cartilage (47.5 vs. 69.8%). Conclusion: Following ACT, VV and SV increased in the repair tissue with time, reflecting maturation of the tissue. One year after the operation, there was a lower level of fibril organization in the repair tissue than in the control cartilage. Thus, the newly synthesized collagen fibrils in the repair tissue appeared to form a denser network than in the control cartilage, but the fibrils remained more randomly oriented. |
Databáze: | OpenAIRE |
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