Ca2+ Ionophore and Phorbol Ester Stimulate Diacylglycerol Formation and Phosphatidylcholine Hydrolysis in Rat Parotid Acinar Cells
| Autor: | Atsushi Yakata, Tetsuya Izawa, Masamichi Noguchi, Kazuhiro Suda, Minoru Tsuboi, Takao Komabayashi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Choline Diglycerides chemistry.chemical_compound Alkaloids Phosphatidylcholine medicine Animals Parotid Gland Staurosporine Ethanolamine Diglyceride Calcimycin Phorbol 12 13-Dibutyrate Phosphocholine Pharmacology Phosphatidylethanolamine Dose-Response Relationship Drug Phospholipase C Phospholipase D Hydrolysis Rats Inbred Strains Rats Biochemistry chemistry Ethanolamines Phosphatidylcholines Phorbol lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Japanese Journal of Pharmacology. 59:97-103 |
| ISSN: | 0021-5198 |
| DOI: | 10.1254/jjp.59.97 |
| Popis: | We investigated the effects of A23187 and phorbol 12,13-dibutyrate (PDBu) on sn-1,2-diacylglycerol (DAG) accumulation and phosphatidylcholine (PC) hydrolysis in rat parotid acinar cells. Both A23187 and PDBu, in concentration ranges of 0.001–0.1 μM, stimulated DAG accumulation and PC hydrolysis in a time- and concentration-dependent manner. Treatment with A23187 and PDBu stimulated the release of [3H]choline and [3H]phosphocholine into the medium, indicating [3H]PC hydrolysis is due to the activation of phospholipases C and D; however, [3H]phosphatidylethanolamine hydrolysis was not indicated. These releases were unaffected by the addition of glucose 6-phosphate, a phosphatase inhibitor. Staurosporine, a protein kinase C inhibitor, significantly inhibited the DAG accumulation and the PC hydrolysis stimulated by these agents. Combinations of A23187 and PDBu potentiated the stimulatory effect which each of these agents alone had on DAG accumulation and PC hydrolysis. This mode of action was additive but not synergistic. These results suggest that DAG accumulation induced by A23187 and PDBu is related to the PC hydrolysis mediated via the activation of phospholipases C and D, and that it is not related to phosphatidylethanolamine hydrolysis. |
| Databáze: | OpenAIRE |
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