Chiral LC method development: Stereo-selective separation, characterization, and determination of cabotegravir and related RS, RR, and SS isomeric impurities on coated cellulose-based chiral stationary phase by HILIC-LC and LC-MS

Autor:	S.R. Krishna Murthy Kasa, Muvvala Venkatanarayana, Lakshmi Narayana Chennuru, B. Chandra Sekhara Rao, Manohar Vemparala, Abdul Fareed Chaman, M.V.N. Kumar Talluri
Rok vydání:	2022
Předmět:	Pharmaceutical Preparations Tandem Mass Spectrometry Clinical Biochemistry Drug Discovery Pharmaceutical Science Stereoisomerism Cellulose Spectroscopy Chromatography High Pressure Liquid Analytical Chemistry Chromatography Liquid
Zdroj:	Journal of pharmaceutical and biomedical analysis. 222
ISSN:	1873-264X
Popis:	The first, sensitive, and rapid chiral method was developed for enatioseperations and determination of cabotegravir and its enantiomeric impurities by using HPLC and LC-MS. Cabotegravir is an antiretroviral medication used for the treatment of HIV/AIDS approved by the food and drugs administration (FDA) in the year 2021. The cabotegravir chiral separation was achieved on the coated cellulose-tris (4-chloro-3-methyl phenyl carbamate) (CHIRALCEL OX-3R) column in HILIC mode and the total run time is less than 15 min. The effects of mobile phase composition, elution mode, and percentage of organic modifier as well as the effect of mobile phase-additives and column temperature were investigated on selectivity, resolution, and peak symmetry. The mobile phase consisted of acetonitrile and water with 0.1% (v/v) addition of formic acid additive with the flow rate of 1 mLmin
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34ac87af5833b4cc609beca321f8e3ed https://pubmed.ncbi.nlm.nih.gov/36194910 Zobrazit plný text záznamu Full Text from ScienceDirect