The B-cell maturation factor Blimp-1 specifies vertebrate slow-twitch muscle fiber identity in response to Hedgehog signaling
| Autor: | Sudipto Roy, Claire Davison, Claire Muxworthy, Christian Wolff, Sarah Baxendale, Philip W. Ingham |
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| Rok vydání: | 2003 |
| Předmět: |
Molecular Sequence Data
Mediator Genetics Animals Myocyte Hedgehog Proteins Hedgehog Transcription factor Zebrafish biology Nuclear Proteins Cell Differentiation Anatomy Zebrafish Proteins biology.organism_classification Embryonic stem cell Hedgehog signaling pathway Cell biology DNA-Binding Proteins Repressor Proteins Slow-Twitch Muscle Fiber Muscle Fibers Slow-Twitch Positive Regulatory Domain I-Binding Factor 1 Signal Transduction Transcription Factors |
| Zdroj: | Nature Genetics. 36:88-93 |
| ISSN: | 1546-1718 1061-4036 |
| DOI: | 10.1038/ng1280 |
| Popis: | Vertebrate skeletal muscles comprise distinct fiber types that differ in their morphology, contractile function, mitochondrial content and metabolic properties. Recent studies identified the transcriptional coactivator PGC-1alpha as a key mediator of the physiological stimuli that modulate fiber-type plasticity in postembryonic development. Although myoblasts become fated to differentiate into distinct kinds of fibers early in development, the identities of regulatory proteins that determine embryonic fiber-type specification are still obscure. Here we show that the gene u-boot (ubo), a mutation in which disrupts the induction of embryonic slow-twitch fibers, encodes the zebrafish homolog of Blimp-1, a SET domain-containing transcription factor that promotes the terminal differentiation of B lymphocytes in mammals. Expression of ubo is induced by Hedgehog (Hh) signaling in prospective slow muscle precursors, and its activity alone is sufficient to direct slow-twitch fiber-specific development by naive myoblasts. Our data provide the first molecular insight into the mechanism by which a specific group of muscle precursors is driven along a distinct pathway of fiber-type differentiation in response to positional cues in the vertebrate embryo. |
| Databáze: | OpenAIRE |
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